Depletion of complement enhances the clearance of Brucella abortus in mice
artículo original
Fecha
2018Autor
González Espinoza, Gabriela
Barquero Calvo, Elías
Lizano González, Esteban
Alfaro Alarcón, Alejandro
Arias Gómez, Berny
Chaves Olarte, Esteban
Lomonte, Bruno
Moreno Robles, Edgardo
Chacón Díaz, Carlos
Metadatos
Mostrar el registro completo del ítemResumen
Brucellosis is a bacterial disease of animals and humans. Brucella abortus
barely activates the innate immune system at the onset of infection, and this
bacterium is resistant to the microbicidal action of complement. Since complement
stands as the first line of defense during bacterial invasions, we explored the role of
complement in B. abortus infections. Brucella abortus-infected mice depleted of complement
with cobra venom factor (CVF) showed the same survival rate as mice in
the control group. The complement-depleted mice readily eliminated B. abortus
from the spleen and did so more efficiently than the infected controls after 7 days
of infection. The levels of the proinflammatory cytokines tumor necrosis factor alpha
and interleukin-6 (IL-6) remained within background levels in complement-depleted
B. abortus-infected mice. In contrast, the levels of the immune activator cytokine
gamma interferon and the regulatory cytokine IL-10 were significantly increased. No
significant histopathological changes in the liver and spleen were observed between
the complement-depleted B. abortus-infected mice and the corresponding controls.
The action exerted by Brucella on the immune system in the absence of complement
may correspond to a broader phenomenon that involves several components
of innate immunity.
External link to the item
10.1128/IAI.00567-18Colecciones
- Microbiología [1171]