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dc.creatorZerpa Catanho, Dessireé Patricia
dc.creatorEsquivel Rodríguez, Patricia
dc.creatorMora Newcomer, Eric
dc.creatorSáenz Murillo, Marco Vinicio
dc.creatorHerrera, Raúl
dc.creatorJiménez García, Víctor
dc.date.accessioned2018-09-06T16:11:06Z
dc.date.available2018-09-06T16:11:06Z
dc.date.issued2017-03
dc.identifier.citationwww.sciencedirect.com/science/article/pii/S0925521416305336es_ES
dc.identifier.issn0925-5214
dc.identifier.urihttp://hdl.handle.net/10669/75589
dc.description.abstractFruit of Carica papaya L. (papaya) has several post-harvest problems mainly caused by quick softening that reduces its shelf life. This softening is directly related to degradation and modification of cell wall oligosaccharides. Therefore, it is important to study, understand and, eventually, regulate the softening process of this fruit to increase its shelf life. This work aims at looking for correlations between transcription patterns of four genes potentially involved in papaya fruit softening with postharvest treatments and the softening process. Papaya fruit (‘Pococí’ hybrid) were treated with ethylene (275–300 mL L−1), 1-methylcyclopropene (1-MCP, 300 nL L−1), or not treated, as control. Fruit were subsequently stored for eleven days at 18–20 °C and 95% relative humidity. During the evaluation period, firmness (N) and color (CIE L*, a* and b*) of pulp and peel were determined; pH, titratable acidity (TA) and total soluble solids (TSS) of pulp were also measured; in addition, transcription patterns of polygalacturonase, endoxylanase, pectinesterase and expansin genes were determined by real time PCR. Treatments showed differences in terms of firmness, color, pH, TA, ripening index and accumulation of transcripts of some genes. Transcription of polygalacturonase and endoxylanase genes correlated negatively with firmness of pulp and peel; whereas pectinesterase gene was positively correlated with peel firmness. No correlation with transcription of the expansin gene analyzed was found. Our results also suggest that polygalacturonase and endoxylanase correlated negatively with papaya fruit firmness and that 1-MCP treatment repressed and reduced the expression of these two genes, respectively. According to these results, silencing genes that encode polygalacturonases or endoxylanases might be a potential strategy to confirm their crucial role on papaya ripening.es_ES
dc.description.sponsorshipFood Security Center of the University of Hohenheim/[]/FSC/Alemaniaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[734-B3-106]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.relation.ispartof
dc.sourcePostharvest Biology and Technology, Vol. 125, pp.42-51es_ES
dc.subjectCarica papayaes_ES
dc.subjectEthylenees_ES
dc.subjectFruit softeninges_ES
dc.subjectGene expressiones_ES
dc.subject1-methylcyclopropenees_ES
dc.subject631.523 3 Genética agrícolaes_ES
dc.titleTranscription analysis of softening-related genes during postharvest of papaya fruit (Carica papaya L. ‘Pococí’ hybrid)es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.date.updated2018-08-09T03:35:40Z
dc.identifier.doi10.1016/j.postharvbio.2016.11.002
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro para Investigaciones en Granos y Semillas (CIGRAS)es_ES
dc.description.procedenceUCR::Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Tecnología de Alimentoses_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Estación Experimental Agrícola Fabio Baudrit Moreno (EEAFBM)es_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro de Investigaciones Agronómicas (CIA)es_ES
dc.identifier.codproyecto734-B3-106


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