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dc.creatorCamacho Matamoros, Natassia
dc.creatorEspinoza Aguirre, Juan Carlos
dc.creatorRodríguez Sánchez, César
dc.creatorRodríguez Cavallini, Evelyn
dc.date.accessioned2018-06-19T20:01:54Z
dc.date.available2018-06-19T20:01:54Z
dc.date.issued2008-03
dc.identifier.citationhttp://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47505-0es_ES
dc.identifier.issn0022-2615
dc.identifier.urihttp://hdl.handle.net/10669/74954
dc.description.abstractTo assess the prevalence of enterotoxigenic Clostridium perfringens among adults suffering from antibiotic-associated diarrhoea in a Costa Rican hospital, faecal samples were analysed from 104 patients by a cultivation approach. The 29 strains obtained, which accounted for an isolation frequency of 28 %, were genotyped and investigated with regard to their in vitro susceptibility to penicillin, imipenem, cefotaxime, chloramphenicol and metronidazole using an agar-dilution method. A multiplex PCR for detection of the toxins α, β and ϵ predictably classified all faecal isolates as biotype A. An agglutination assay revealed that only one isolate synthesized detectable amounts of enterotoxin (detection rate 3 %). This result was confirmed by a PCR targeting the cpe gene. The spores of the only CPE+ isolate did not germinate after incubation for 30 min at temperatures above 80 °C. Most isolates were susceptible to first-choice antimicrobials. However, unusual MICs for penicillin (16 μg ml−1) and metronidazole (512 μg ml−1) were detected in one and three isolates, respectively. The low incidence of enterotoxigenic strains suggests that C. perfringens was not a major primary cause of antibiotic-associated diarrhoea in this hospital during the sampling period.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-A5-027]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Medical Microbiology, Vol 57, pp 343–347es_ES
dc.subjectCPEes_ES
dc.subjectClostridium perfringens enterotoxines_ES
dc.subjectAADes_ES
dc.subjectAntibiotic-associated diarrhoeaes_ES
dc.subject616.342 7 Diarreaes_ES
dc.titleIsolates of Clostridium perfringens recovered from Costa Rican patients with antibiotic-associated diarrhoea are mostly enterotoxin-negative and susceptible to first-choice antimicrobialses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.date.updated2018-05-21T21:55:36Z
dc.identifier.doi10.1099/jmm.0.47505-0
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Docencia::Salud::Facultad de Microbiologíaes_ES
dc.identifier.codproyecto803-A5-027


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