Show simple item record

dc.creatorMoreira Soto, Rolando Daniel
dc.creatorMoreira Soto, Andrés
dc.creatorCorrales Aguilar, Eugenia
dc.creatorCalderón Arguedas, Ólger
dc.creatorTroyo Rodríguez, Adriana
dc.date.accessioned2018-05-28T21:15:47Z
dc.date.available2018-05-28T21:15:47Z
dc.date.issued2017-06
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S1877959X17301115?via%3Dihub#!es_ES
dc.identifier.issn1877-959X
dc.identifier.urihttp://hdl.handle.net/10669/74775
dc.description.abstractRickettsiae are intracellular bacteria commonly associated with hematophagous arthropods. Most of them have been described in hard ticks, but some have been found in soft ticks. Here we report the detection and isolation of a new Rickettsia from Ornithodoros knoxjonesi larvae collected from Balantiopteryx plicata (Emballonuridae) in Nicoya, Costa Rica. Two ticks were processed to detect Rickettsia spp. genes gltA, ompA, ompB, and htrA by PCR. Part of the macerate was also inoculated into Vero E6 and C6/36 cell lines, and cells were evaluated by Giménez stain, indirect immunofluorescence assay (IFA), and PCR. Both ticks were positive by PCR and rickettsial growth was successful in Vero E6 cells. Amplification and sequencing of near full length rrs, gltA, sca4 genes, and fragments of ompA and ompB showed that the Rickettsia sp. was different from described species. The highest homologies were with ‘Candidatus Rickettsia wissemanii’ and Rickettsia peacockii: 99.70% (1321/1325) with both sequences for rrs, 99.58% (1172/1177) and 99.76% (1246/1249) for gltA, 99.26% with both sequences (2948/2970 and 2957/2979) for sca4, 98.78% (485/491) and 98.39% (2069/2115) for ompA, and 98.58 (1453/1474) and 98.92% (1459/1475) for ompB; respectively. Bat blood, spleen, liver, and lung samples analyzed for Rickettsia detection were negative. Results demonstrate that the Rickettsia isolated from O. knoxjonesi is probably an undescribed species that belongs to the spotted fever group, for which ‘Candidatus Rickettsia nicoyana’ is proposed. Considering that B. plicata inhabits areas where contact with humans may occur and that human parasitism by Ornithodoros has been reported in the country, it will be important to continue with the characterization of this species and its pathogenic potential.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-B4-055 ]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-B4-656]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.relation.ispartofTicks and Tick-borne Diseases Volumen 8 Número 4
dc.sourceTicks and Tick-borne Diseases. Vol 8. pp 532-536es_ES
dc.subjectRickettsialeses_ES
dc.subjectArgasidaees_ES
dc.subjectEmballonuridaees_ES
dc.subjectBalantiopteryxes_ES
dc.subjectCosta Ricaes_ES
dc.subject579.327 728 66 Rickettsias y Chlamydiases_ES
dc.title‘Candidatus Rickettsia nicoyana’: A novel Rickettsia species isolated from Ornithodoros knoxjonesi in Costa Ricaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.date.updated2018-05-15T21:35:02Z
dc.identifier.doi10.1016/j.ttbdis.2017.02.015
dc.description.procedenceUCR::Docencia::Salud::Facultad de Microbiologíaes_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Estructuras Microscópicas (CIEMIC)es_ES
dc.identifier.codproyecto803-B4-656
dc.identifier.codproyecto803-B4-055


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record