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dc.creatorEngmark, Mikael Gerling
dc.creatorAndersen, Mikael Rørdam
dc.creatorLaustsen, Andreas Hougaard
dc.creatorPatel, Jigar
dc.creatorSullivan, Eric
dc.creatorde Masi, Federico
dc.creatorHansen, Christian S.
dc.creatorKringelum, Jens V.
dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.creatorLund, Ole
dc.date.accessioned2018-05-18T14:22:34Z
dc.date.available2018-05-18T14:22:34Z
dc.date.issued2016-11-08
dc.identifier.citationhttps://www.nature.com/articles/srep36629es_ES
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10669/74732
dc.description.abstractSnakebite envenoming is a serious condition requiring medical attention and administration of antivenom. Current antivenoms are antibody preparations obtained from the plasma of animals immunised with whole venom(s) and contain antibodies against snake venom toxins, but also against other antigens. In order to better understand the molecular interactions between antivenom antibodies and epitopes on snake venom toxins, a high-throughput immuno-profiling study on all manually curated toxins from Dendroaspis species and selected African Naja species was performed based on custom-made high-density peptide microarrays displaying linear toxin fragments. By detection of binding for three different antivenoms and performing an alanine scan, linear elements of epitopes and the positions important for binding were identified. A strong tendency of antivenom antibodies recognizing and binding to epitopes at the functional sites of toxins was observed. With these results, high-density peptide microarray technology is for the first time introduced in the field of toxinology and molecular details of the evolution of antibody-toxin interactions based on molecular recognition of distinctive toxic motifs are elucidated.es_ES
dc.description.sponsorshipNovo Nordisk Foundation/[13OC0005613]/NNF/Dinamarcaes_ES
dc.description.sponsorshipNovo Nordisk Foundation/[16OC0019248]/NNF/Dinamarcaes_ES
dc.language.isoen_USes_ES
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.sourceScientific Reports, vol. 6, 36629es_ES
dc.subjectEpitope mappinges_ES
dc.subjectMicroarrayes_ES
dc.subjectDendroaspises_ES
dc.subjectSnake venomes_ES
dc.subjectAntivenomes_ES
dc.titleHigh-throughput immuno-profiling of mamba (Dendroaspis) venom toxin epitopes using high-density peptide microarrayses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1038/srep36629
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.identifier.pmid27824133
dc.identifier.pmidPMC5100549


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CC0 1.0 Universal
Except where otherwise noted, this item's license is described as CC0 1.0 Universal