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dc.creatorEngmark, Mikael Gerling
dc.creatorJespersen, Martin Closter
dc.creatorLomonte, Bruno
dc.creatorLund, Ole
dc.creatorLaustsen, Andreas Hougaard
dc.date.accessioned2018-05-17T21:43:58Z
dc.date.available2018-05-17T21:43:58Z
dc.date.issued2017-11
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0041010117302738?via%3Dihub#!es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/74730
dc.description.abstractPolyvalent snakebite antivenoms derive their therapeutic success from the ability of their antibodies to neutralize venom toxins across multiple snake species. This ability results from a production process involving immunization of large mammals with a broad suite of toxins present in venoms. As a result of immunization with this wide range of toxins, many polyvalent antivenoms have a high degree of crossreactivity to similar toxins in other snake venoms e a cross-reactivity which cannot easily be deconvoluted. As a proof of concept, we aimed at exploring the opposite scenario by performing a highthroughput evaluation of the extent of cross-reactivity of a polyclonal mixture of antibodies that was raised against only a single snake venom fraction. For this purpose, a venom fraction containing short neurotoxin 1 (SN-1; Uniprot accession number P01416, three-finger toxin (3FTx) family), which is the medically most important toxin from the notorious black mamba (Dendroaspis polylepis), was employed. Following immunization of a rabbit, a specific polyclonal antibody response was confirmed by ELISA and immunodiffusion. Subsequently, these antibodies were investigated by high-density peptide microarray to reveal linear elements of recognized epitopes across 742 3FTxs and 10 dendrotoxins. This exploratory study demonstrates in a single immunized animal that cross-reactivity between toxins of high similarity may be difficult to obtain when immunizing with a single 3FTx containing venom fraction. Additionally, this study explored the influence of employing different lengths of peptides in high-density peptide microarray experiments for identification of toxin epitopes. Using 8-mer, 12-mer, and 15-mer peptides, a single linear epitope element was identified in SN-1 with high precision.es_ES
dc.description.sponsorshipNovo Nordisk Fonden/[NNF13OC0005613]/NNF/Dinamarcaes_ES
dc.description.sponsorshipNovo Nordisk Fonden/[NNF16OC0019248]/NNF/Dinamarcaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon, Vol 138, pp 151-158es_ES
dc.subjectEpitope mappinges_ES
dc.subjectSingle toxin immunizationes_ES
dc.subjectThree-finger toxines_ES
dc.subjectDendroaspis polylepises_ES
dc.subjectShort neurotoxines_ES
dc.subjectSnake venomes_ES
dc.titleHigh-density peptide microarray exploration of the antibody response in a rabbit immunized with a neurotoxic venom fractiones_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2017.08.028
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Docencia::Salud::Facultad de Microbiologíaes_ES
dc.identifier.pmid28867663


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