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dc.creatorYunes Quartino, Pablo J.
dc.creatorPortela, Madelón
dc.creatorLima, Analía
dc.creatorDurán, Rosario
dc.creatorLomonte, Bruno
dc.creatorFidelio, Gerardo Daniel
dc.date.accessioned2018-05-02T15:43:19Z
dc.date.available2018-05-02T15:43:19Z
dc.date.issued2015-10
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0005273615001819?via%3Dihubes_ES
dc.identifier.issn0005-2736
dc.identifier.urihttp://hdl.handle.net/10669/74581
dc.description.abstractWe present an analysis of lipid monolayer hydrolysis at a constant area to assess the optimal lateral surface pressure value (Πopt) and thus, the surface packing density of the lipid, at which the activity of a given lipolytic enzyme is maximal. This isochoric method consists of a measurement of the decrease down to zero of theΠopt of phospholipid substrate monolayer due to continuous hydrolysis using only one reaction compartment. We performed the comparison of both approaches using several commercially available and literature-evaluated sPLA2s. Also, we characterized for the first time the profile of hydrolysis of DLPC monolayers catalyzed by a sPLA2 from Streptomyces violaceoruber and isoenzymes purified from Bothrops diporus venom. One of these viper venomenzymes is a newisoenzyme, partially sequenced by amass spectrometry approach.We also included the basicmyotoxin sPLA2-III fromBothrops asper. Results obtained with the isochoricmethod and the standard isobaric one produced quite similar values of Πopt, validating the proposal. In addition, we propose a new classification parameter, a lipolytic ratio of hydrolysis at two lateral pressures, 20 mN·m−1 and 10 mN·m−1, termed here as LR20/10 index. This index differentiates quite well “high surface pressure” from “low surface pressure” sPLA2s and, by extension; it can be used as a functional criterion for the quality of a certain enzyme. Also, this index could be added to the grouping systematic criteria for the superfamily proposed for phospholipase A2.es_ES
dc.description.sponsorshipLa Secretaría de Ciencia y Tecnología/[2014-2015, Res. 103/15]/SECyT-UNC/Argentinaes_ES
dc.description.sponsorshipFondo para la Investigación Científica y Tecnológica/[BID PICT 2011-1784]/FONCYT-MinCyT/Argentinaes_ES
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas/[PIP 2012-2014]/CONICET/Argentinaes_ES
dc.description.sponsorshipInternational Centre for Genetic Engineering and Biotechnology/[ICGEB CRP/COS13-01]/ICGEB/Italiaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B4-100]/UCR/Costa Ricaes_ES
dc.description.sponsorshipIbero-American Network CYTED BIOTOX/[Red Biotox 212RT0467]/CYTED BIOTOX/Argentinaes_ES
dc.language.isoen_USes_ES
dc.sourceBiochimica et Biophysica Acta-Biomembranes, vol. 1848, núm. 10, part A, 2216-2224 (2015)es_ES
dc.subjectLipid monolayeres_ES
dc.subjectPhospholipase A2es_ES
dc.subjectSurface pressurees_ES
dc.subjectIsochoric methodes_ES
dc.subjectBothrops diporus sPLA2es_ES
dc.subjectBothrops asper myotoxines_ES
dc.subjectSnake venomes_ES
dc.titleA constant area monolayer method to assess optimal lipid packing for lipolysis tested with several secreted phospholipases A2es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.bbamem.2015.06.003
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-B4-100
dc.identifier.pmid26051123


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