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dc.creatorDeuschle, M.
dc.creatorLimbrunner, S.
dc.creatorRother, D.
dc.creatorWahler, S.
dc.creatorChavarría Vargas, Max
dc.creatorde Lorenzo, Víctor
dc.creatorKremling, Andreas
dc.creatorPflüger Grau, Katharina
dc.date.accessioned2018-04-20T20:05:06Z
dc.date.available2018-04-20T20:05:06Z
dc.date.issued2015-08-27
dc.identifier.citationhttp://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12323/epdfes_ES
dc.identifier.issn1758-2229
dc.identifier.other1758-2229
dc.identifier.urihttp://hdl.handle.net/10669/74501
dc.description.abstractThe nitrogen phosphotransferase system (PTSNtr) of Pseudomonas putida is a multi-component regulatory device that participates in controlling a variety of physiological processes in a post-translational fashion. A general survey of genes regulated by PtsN exposed transcription of the kdpFABC operon is most conspicuously affected. Measurements of kdpFp promoter activity in different pts mutants showed that PtsN is responsible for repression of kdpFABC transcription. This effect could be assigned mainly to PtsN∼P, depending on the external K+ concentration. Bacterial two-hybrid assays demonstrated that kdpFp regulation is implemented through direct interaction of the PtsN protein with the sensor kinase KdpD of the KdpD/KdpE two-component system. Interaction between KdpD and PtsN was detectable with a PtsN variant that imitates the non-phosphorylated form as well as with a PtsN type mimicking the phosphorylated form of PtsN. These results raise a regulatory scenario in which the Kdp system is regulated by the action of PtsN through direct interaction with the sensor kinase KdpD, and the outcome of such an interaction depends on the phosphorylation state of PtsN as well as on the external K+ concentration.es_ES
dc.description.sponsorshipDeutsche Forschungsgemeinschaft/[PF 711/2-1]/DFG/Alemaniaes_ES
dc.language.isoen_USes_ES
dc.sourceEnvironmental microbiology reports, Vol. 7(6), 2015es_ES
dc.subjectPseudomonas putidaes_ES
dc.subjectProteines_ES
dc.subjectkdpFABC operones_ES
dc.subjectPtsNes_ES
dc.subjectkdpFp activityes_ES
dc.subjectK + uptakees_ES
dc.titleInterplay of the PtsN (EIIANtr) protein of Pseudomonas putida with its target sensor kinase KdpDes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1111/1758-2229.12323
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Químicaes_ES
dc.identifier.pmid26224366


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