dc.creator | Rey Suárez, Paola | |
dc.creator | Floriano, Rafael Stuani | |
dc.creator | Rostelato Ferreira, Sandro | |
dc.creator | Saldarriaga Córdoba, Mónica María | |
dc.creator | Núñez Rangel, Vitelbina | |
dc.creator | Rodrigues Simioni, Lea | |
dc.creator | Lomonte, Bruno | |
dc.date.accessioned | 2018-04-20T13:43:33Z | |
dc.date.available | 2018-04-20T13:43:33Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | https://www.sciencedirect.com/science/article/pii/S0041010112004990 | |
dc.identifier.issn | 0041-0101 | |
dc.identifier.uri | https://hdl.handle.net/10669/74496 | |
dc.description.abstract | The major venom component of Micrurus mipartitus, a coral snake distributed from
Nicaragua to northern South America, was characterized biochemically and functionally.
This protein, named mipartoxin-I, is a novel member of the three-finger toxin superfamily,
presenting the characteristic cysteine signature and amino acid sequence length of the
short-chain, type-I, a-neurotoxins. Nevertheless, it varies considerably from related toxins,
with a sequence identity not higher than 70% in a multiple alignment of 67 proteins within
this family. Its observed molecular mass (7030.0) matches the value predicted by its amino
acid sequence, indicating lack of post-translational modifications. Mipartoxin-I showed
a potent lethal effect in mice (intraperitoneal median lethal dose: 0.06 mg/g body weight),
and caused a clear neuromuscular blockade on both avian and mouse nerve-muscle
preparations, presenting a post-synaptic action through the cholinergic nicotinic receptor.
Since mipartoxin-I is the most abundant (28%) protein in M. mipartitus venom, it should
play a major role in its toxicity, and therefore represents an important target for developing
a therapeutic antivenom, which is very scarce or even unavailable in the regions where this
snake inhabits. The structural information here provided might help in the preparation of
a synthetic or recombinant immunogen to overcome the limited venom availability. | es_ES |
dc.description.sponsorship | Departamento Administrativo de Ciencia, Tecnología e Innovación/[1115-459-21441]/COLCIENCIAS/Colombia | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[741-A9-513]/UCR/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicon, 60(5), 851-863 (2012) | es_ES |
dc.subject | Micrurus | es_ES |
dc.subject | mipartoxin | es_ES |
dc.subject | coral snake | es_ES |
dc.subject | Snake venom | es_ES |
dc.title | Mipartoxin-I, a novel three-finger toxin, is the major neurotoxic component in the venom of the redtail coral snake Micrurus mipartitus (Elapidae) | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/j.toxicon.2012.05.023 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.identifier.codproyecto | 741-A9-513 | |