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dc.creatorRey Suárez, Paola
dc.creatorStuani Floriano, Rafael
dc.creatorRostelato Ferreira, Sandro
dc.creatorSaldarriaga Córdoba, Mónica María
dc.creatorNúñez Rangel, Vitelbina
dc.creatorRodrigues Simioni, Lea
dc.creatorLomonte, Bruno
dc.date.accessioned2018-04-20T13:43:33Z
dc.date.available2018-04-20T13:43:33Z
dc.date.issued2012
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0041010112004990es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/74496
dc.description.abstractThe major venom component of Micrurus mipartitus, a coral snake distributed from Nicaragua to northern South America, was characterized biochemically and functionally. This protein, named mipartoxin-I, is a novel member of the three-finger toxin superfamily, presenting the characteristic cysteine signature and amino acid sequence length of the short-chain, type-I, a-neurotoxins. Nevertheless, it varies considerably from related toxins, with a sequence identity not higher than 70% in a multiple alignment of 67 proteins within this family. Its observed molecular mass (7030.0) matches the value predicted by its amino acid sequence, indicating lack of post-translational modifications. Mipartoxin-I showed a potent lethal effect in mice (intraperitoneal median lethal dose: 0.06 mg/g body weight), and caused a clear neuromuscular blockade on both avian and mouse nerve-muscle preparations, presenting a post-synaptic action through the cholinergic nicotinic receptor. Since mipartoxin-I is the most abundant (28%) protein in M. mipartitus venom, it should play a major role in its toxicity, and therefore represents an important target for developing a therapeutic antivenom, which is very scarce or even unavailable in the regions where this snake inhabits. The structural information here provided might help in the preparation of a synthetic or recombinant immunogen to overcome the limited venom availability.es_ES
dc.description.sponsorshipDepartamento Administrativo de Ciencia, Tecnología e Innovación/[1115-459-21441]/COLCIENCIAS/Colombiaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A9-513]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon, 60(5), 851-863 (2012)es_ES
dc.subjectMicruruses_ES
dc.subjectmipartoxines_ES
dc.subjectcoral snakees_ES
dc.subjectSnake venomes_ES
dc.titleMipartoxin-I, a novel three-finger toxin, is the major neurotoxic component in the venom of the redtail coral snake Micrurus mipartitus (Elapidae)es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2012.05.023
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-A9-513


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