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dc.creatorAzofeifa Chinchilla, Karol
dc.creatorAngulo Ugalde, Yamileth
dc.creatorLomonte, Bruno
dc.date.accessioned2018-03-09T16:14:53Z
dc.date.available2018-03-09T16:14:53Z
dc.date.issued2008
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0041010107003819es_ES
dc.identifier.issn0010-4140
dc.identifier.urihttp://hdl.handle.net/10669/74288
dc.description.abstractFucoidan, a natural polysaccharide extracted from brown seaweed, inhibits the myotoxic phospholipases A2 present in the venoms of crotalid snakes. This study evaluated the influence of molecular weight on the ability of fucoidan to prevent muscle necrosis when rapidly administered after injection of a purified myotoxin or crude venom of Bothrops asper, in a mouse model. It was hypothesized that smaller fucoidan fragments, being of higher diffusibility to tissues, might have a better neutralizing efficiency in vivo. Fucoidan was subjected to acid hydrolysis to obtain low-molecular weight fragments (FL), or to gel filtration to isolate its high-molecular weight fraction (FH). These two preparations were standardized to the same neutralizing potency by preincubation assays, and subsequently tested in vivo, by independent administration assays. Local i.m. administration of either FH or FL, immediately after i.m. injection of myotoxin II, prevented nearly 50% of muscle necrosis, albeit with no difference between the two preparations. Muscle necrosis was not reduced when either FH or FL was administered by i.v. route, immediately after i.m. toxin injection. When tested against crude venom, which contains several myotoxin isoforms, the immediate in situ i.m. injection of FH still inhibited myonecrosis by nearly one-half of the effect recorded in the untreated group, whereas FL was ineffective. It is concluded that, in this model, and in contrast to expectations, the use of smaller fucoidan fragments to prevent muscle damage induced by snake venom myotoxins is not advantageous, when compared with larger fucoidan molecules.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A4-070]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon, vol. 51, 373-380 (2008)es_ES
dc.subjectMyonecrosises_ES
dc.subjectMyotoxines_ES
dc.subjectFucoidanes_ES
dc.subjectPhospholipase A2es_ES
dc.subjectSnake venomes_ES
dc.titleAbility of fucoidan to prevent muscle necrosis induced by snake venom myotoxins: comparison of high- and low-molecular weight fractionses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2007.10.008
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-A4-070
dc.identifier.pmid18061642


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