Show simple item record

dc.creatorDíaz Oreiro, Cecilia
dc.creatorLomonte, Bruno
dc.creatorZamudio, Fernando
dc.creatorGutiérrez, José María
dc.date.accessioned2017-09-26T20:12:53Z
dc.date.available2017-09-26T20:12:53Z
dc.date.issued1995
dc.identifier.citationhttp://onlinelibrary.wiley.com/doi/10.1002/nt.2620030107/fulles_ES
dc.identifier.issn1522-7189
dc.identifier.urihttp://hdl.handle.net/10669/73297
dc.description.abstractA basic myotoxic protein was purified from Bothrops asper venom. Like other basic Bothrops myotoxins, myotoxin IV induces acute muscle damage after intramuscular injection in mice and disrupts negatively charged liposomes but not positively charged ones. Furthermore, this protein exerts a weak anticoagulant effect only at concentrations of 40 μg/ml or higher, and is devoid of phospholipase A2 activity. Rabbit polyclonal antibodies to B. asper myotoxin II, a related lysine-49 isoform, cross-react strongly with myotoxin IV by enzyme immunoassay, indicating a high degree of antigenic similarity between these two proteins. The fact that both toxins have similar amino acid compositions and amino-terminal sequences, including leucine-5 and glutamine-11, 2 amino acid residues conserved in all lysine-49 phospholipase A2 variants purified, strongly suggests that B. asper myotoxin IV is a lysine-49 phospholipase A2.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[Project 741-90-044]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceNatural Toxins, Vol. 3, Núm. 1, 1995es_ES
dc.subjectSnake venomes_ES
dc.subjectMyotoxines_ES
dc.subjectPhospholipases A2es_ES
dc.subjectLys-49 variantes_ES
dc.subjectMyonecrosises_ES
dc.titlePurification and characterization of myotoxin IV, a phospholipase A2 variant, from Bothrops asper snake venomes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1002/nt.2620030107
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Docencia::Salud::Facultad de Microbiologíaes_ES
dc.identifier.pmid7749580


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record