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Molecular characterization and genetic diversity of Jatropha curcas L. in Costa Rica
dc.creator | Vásquez Mayorga, Marcela | |
dc.creator | Fuchs Castillo, Eric J. | |
dc.creator | Hernández Jiménez, Eduardo José | |
dc.creator | Herrera Murillo, Franklin | |
dc.creator | Hernández López, Jesús | |
dc.creator | Moreira González, Ileana | |
dc.creator | Arnáez Serrano, Elizabeth | |
dc.creator | Barboza Vargas, Natalia María | |
dc.date.accessioned | 2017-07-26T20:04:36Z | |
dc.date.available | 2017-07-26T20:04:36Z | |
dc.date.issued | 2017-02-09 | |
dc.identifier.citation | https://peerj.com/articles/2931/ | |
dc.identifier.issn | 2167-8359 | |
dc.identifier.other | PMC5345823 | |
dc.identifier.uri | https://hdl.handle.net/10669/30396 | |
dc.description.abstract | We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was evaluated using G-SSRs and SCARs markers. A Neighbor-Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He = 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC = 0.274) and inbreeding coefficient (f = − 0.102) were both low. Clustering was not related to the geographical origin of accessions. International accessions clustered independently of collection sites, suggesting a lack of genetic structure, probably due to the wide distribution of this crop and ample gene flow. Molecular markers identified only one non-toxic accession (JCCR-24) from Mexico. This work is part of a countrywide effort to characterize the genetic diversity of the Jatropha curcas germplasm bank in Costa Rica. | es_ES |
dc.description.sponsorship | Consejo Nacional de Rectores/[736-B1-660]/CONARE/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.rights | Atribución 3.0 Costa Rica | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/cr/ | * |
dc.source | Peerj; 2017 | es_ES |
dc.subject | Genetic diversity | es_ES |
dc.subject | Jatropha curcas | es_ES |
dc.subject | SCARs markers | es_ES |
dc.subject | nrDNA-ITS region | es_ES |
dc.subject | EST-SSR | es_ES |
dc.title | Molecular characterization and genetic diversity of Jatropha curcas L. in Costa Rica | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.7717/peerj.2931 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM) | es_ES |
dc.identifier.pmid | 28289556 |
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Biología [1644]