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dc.creatorLeite Costa, Cecília
dc.creatorLópez Ureña, Diana
dc.creatorde Oliveira Assis, Thiago
dc.creatorRibeiro, Ronaldo A.
dc.creatorSilveira Silva, Rodrigo Otavio
dc.creatorRupnik, Maja
dc.creatorWilcox, Mark H.
dc.creatorFiorini de Carvalho, Alex
dc.creatorOliveira do Carmo, Anderson
dc.creatorAbalen Martins Dias, Adriana
dc.creatorBarreto Mano de Carvalho, Cibele
dc.creatorChaves Olarte, Esteban
dc.creatorRodríguez Sánchez, César
dc.creatorQuesada Gómez, Carlos
dc.creatorde Castro Brito, Gerly Anne
dc.date.accessioned2017-07-19T15:29:36Z
dc.date.available2017-07-19T15:29:36Z
dc.date.issued2016-08
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S1075996416300737es_ES
dc.identifier.issn1075-9964
dc.identifier.urihttp://hdl.handle.net/10669/30377
dc.description.abstractThe epidemiology of Clostridium difficile infections is highly dynamic as new strains continue to emerge worldwide. Here we present a detailed analysis of a new C. difficile strain (ICC-45) recovered from a cancer patient in Brazil that died from severe diarrhea. A polyphasic approach assigned a new PCR-ribotype and PFGE macrorestriction pattern to strain ICC-45, which is toxigenic (tcdA+, tcdB+ and ctdB+) and classified as ST41 from MLST Clade 2 and toxinotype IXb. Strain ICC-45 encodes for a variant TcdB that induces a distinct CPE in agreement with its toxinotype. Unlike epidemic NAP1/027 strains, which are also classified to MLST Clade 2, strain ICC-45 is susceptible to fluoroquinolones and does not overproduce toxins TcdA and TcdB. However, supernatants from strain ICC-45 and a NAP1/027 strain produced similar expression of pro-inflammatory cytokines, epithelial damage, and oxidative stress response in the mouse ileal loop model. These results highlight inflammation and oxidative stress as common features in the pathogenesis of C. difficile Clade 2 strains. Finally, this work contributes to the description of differences in virulence among various C. difficile strains.es_ES
dc.description.sponsorshipCoordenação de aperfeiçoamento de pessoal de nivel superior y Conselho Nacional de Desenvolvimento Científico e Tecnológico/[CI-1-00830-00061.01.00/13]/FUNCAP-CAPES and PPSUS-REDEMS-CNPq-FUNCAP-SESA/Brasiles_ES
dc.description.sponsorshipCoordenação de aperfeiçoamento de pessoal de nivel superior y Conselho Nacional de Desenvolvimento Científico e Tecnológico/[12535679-0]/FUNCAP-CAPES and PPSUS-REDEMS-CNPq-FUNCAP-SESA/Brasiles_ES
dc.description.sponsorshipMinisterio de Ciencia, Tecnología y Telecomunicaciones y Consejo Nacional de Rectores/[803-B1-654]/MICITT-CONARE/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Ciencia, Tecnología y Telecomunicaciones y Consejo Nacional de Rectores/[803-B4-652]/MICITT-CONARE/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-B5-107]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-B5-108]/UCR/Costa Ricaes_ES
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología/[FV-0004-13]/CONICIT/Costa Rica. Fondo gestionado a través de FORINVESes_ES
dc.language.isoen_USes_ES
dc.sourceAnaerobe; Volume 40. 2016es_ES
dc.subjectClostridium difficilees_ES
dc.subjectMLST Clade 2es_ES
dc.subjectVariant toxin Bes_ES
dc.subjectInflammationes_ES
dc.subjectOxidative stress responsees_ES
dc.titleA MLST Clade 2 Clostridium difficile strain with a variant TcdB induces severe inflammatory and oxidative response associated with mucosal disruptiones_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.anaerobe.2016.06.005
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.identifier.pmid27311833


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