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dc.creatorAngulo Ugalde, Yamileth
dc.creatorCastro Godínez, Adriana
dc.creatorLomonte, Bruno
dc.creatorRucavado Romero, Alexandra
dc.creatorFernández Ulate, Julián
dc.creatorCalvete Chornet, Juan José
dc.creatorGutiérrez, José María
dc.date.accessioned2017-07-07T16:55:27Z
dc.date.available2017-07-07T16:55:27Z
dc.date.issued2014-12
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S030090841400296Xes_ES
dc.identifier.issn0300-9084
dc.identifier.urihttp://hdl.handle.net/10669/30339
dc.description.abstractFour disintegrins were isolated from the venoms of the Central American viperid snakes Atropoides mexicanus (atropoimin), Bothrops asper (bothrasperin), Cerrophidion sasai (sasaimin), and Crotalus simus (simusmin). Purifications were performed by reverse-phase HPLC. The four disintegrins have biochemical characteristics, i.e. molecular mass and location of Cys, which allow their classification within the group of medium-size disintegrins. All of them present the canonical RGD sequence, which determines their interaction with integrins in cell membranes. The disintegrins inhibited ADP and collagen-induced human platelet aggregation, with similar IC50s in the nM range. In addition, disintegrins inhibited the adhesion of an endothelial cell line and a melanoma cell line to the extracellular matrix proteins type I collagen, laminin, fibronectin, and vitronectin, albeit showing variable ability to exert this activity. This study expands the inventory of this family of viperid venom proteins, and reports, for the first time, disintegrins from the venoms of species of the genera Atropoides and Cerrophidion.es_ES
dc.description.sponsorshipConsejo Nacional de Rectores/[741-A9-506]/CONARE-FEES/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A8-044]/UCR/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovaci on/[BFU2010-1737]//Españaes_ES
dc.language.isoen_USes_ES
dc.sourceBiochimie; Volumen 107, Part B. 2014es_ES
dc.subjectDisintegrinses_ES
dc.subjectSnake venomes_ES
dc.subjectPlatelet aggregationes_ES
dc.subjectCell adhesiones_ES
dc.subjectIntegrinses_ES
dc.titleIsolation and characterization of four medium-size disintegrins from the venoms of Central American viperid snakes of the genera Atropoides, Bothrops, Cerrophidion and Crotaluses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.biochi.2014.10.010
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid25457103


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