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dc.creatorLaustsen, Andreas Hougaard
dc.creatorGutiérrez, José María
dc.creatorRasmussen, Arne R.
dc.creatorEngmark, Mikael Gerling
dc.creatorGravlund, Peter
dc.creatorSanders, Kate L.
dc.creatorLohse, Brian
dc.creatorLomonte, Bruno
dc.date.accessioned2017-07-03T16:59:12Z
dc.date.available2017-07-03T16:59:12Z
dc.date.issued2015-12-01
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010115300179es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/30171
dc.description.abstractFour specimens of the olive sea snake, Aipysurus laevis, were collected off the coast of Western Australia, and the venom proteome was characterized and quantitatively estimated by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses. A. laevis venom is remarkably simple and consists of phospholipases A2 (71.2%), three-finger toxins (3FTx; 25.3%), cysteine-rich secretory proteins (CRISP; 2.5%), and traces of a complement control module protein (CCM; 0.2%). Using a Toxicity Score, the most lethal components were determined to be short neurotoxins. Whole venom had an intravenous LD50 of 0.07 mg/kg in mice and showed a high phospholipase A2 activity, but no proteinase activity in vitro. Preclinical assessment of neutralization and ELISA immunoprofiling showed that BioCSL Sea Snake Antivenom was effective in cross-neutralizing A. laevis venom with an ED50 of 821 μg venom per mL antivenom, with a binding preference towards short neurotoxins, due to the high degree of conservation between short neurotoxins from A. laevis and Enhydrina schistosa venom. Our results point towards the possibility of developing recombinant antibodies or synthetic inhibitors against A. laevis venom due to its simplicity.es_ES
dc.description.sponsorshipNovo Nordisk Fonden/[NNF13OC0005613]//Dinamarcaes_ES
dc.description.sponsorshipDansk Tennis Fond Oticon Fonden/[14-0857]//Dinamarcaes_ES
dc.description.sponsorshipLundbeckfonden/[R169-2014-833]//Dinamarcaes_ES
dc.description.sponsorshipFonden for Lægevidenskabens Fremme/[14-253]//Dinamarcaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 107, Parte B. 2015es_ES
dc.subjectAipysurus laevises_ES
dc.subjectOlive sea snakees_ES
dc.subjectSnake venomes_ES
dc.subjectProteomicses_ES
dc.subjectToxicityes_ES
dc.subjectVenomicses_ES
dc.titleDanger in the reef: Proteome, toxicity, and neutralization of the venom of the olive sea snake, Aipysurus laevises_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2015.07.008
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid26169672


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