Mostrar el registro sencillo del ítem
Lemnitoxin, the major component of Micrurus lemniscatus coral snake venom, is a myotoxic and pro-inflammatory phospholipase A2
dc.creator | Casais e Silva, Luciana Lyra | |
dc.creator | Teixeira, Catarina de Fátima | |
dc.creator | Lebrun, Ivo | |
dc.creator | Lomonte, Bruno | |
dc.creator | Alape Girón, Alberto | |
dc.creator | Gutiérrez, José María | |
dc.date.accessioned | 2017-06-26T21:35:15Z | |
dc.date.available | 2017-06-26T21:35:15Z | |
dc.date.issued | 2016-08-26 | |
dc.identifier.citation | http://www.sciencedirect.com/science/journal/03784274 | |
dc.identifier.issn | 0378-4274 | |
dc.identifier.uri | https://hdl.handle.net/10669/30161 | |
dc.description.abstract | The venom of Micrurus lemniscatus, a coral snake of wide geographical distribution in South America, was fractionated by reverse-phase HPLC and the fractions screened for phospholipase A2 (PLA2) activity. The major component of the venom, a PLA2, here referred to as ‘Lemnitoxin’, was isolated and characterized biochemically and toxicologically. It induces myotoxicity upon intramuscular or intravenous injection into mice. The amino acid residues Arg15, Ala100, Asn108, and a hydrophobic residue at position 109, which are characteristic of myotoxic class I phospholipases A2, are present in Lemnitoxin. This PLA2 is antigenically related to M. nigrocinctus nigroxin, Notechis scutatus notexin, Pseudechis australis mulgotoxin, and Pseudonaja textilis textilotoxin, as demonstrated with monoclonal and polyclonal antibodies. Lemnitoxin is highly selective in its targeting of cells, being cytotoxic for differentiated myotubes in vitro and muscle fibers in vivo, but not for undifferentiated myoblasts or endothelial cells. Lemnitoxin is not lethal after intravenous injection at doses up to 2 μg/g in mice, evidencing its lack of significant neurotoxicity. Lemnitoxin displays anticoagulant effect on human plasma and proinflammatory activity also, as it induces paw edema and mast cell degranulation. Thus, the results of this work demonstrate that Lemnitoxin is a potent myotoxic and proinflammatory class I PLA2. | es_ES |
dc.description.sponsorship | International Centre for Genetic Engineering and Biotechnology/[CRP/COS13-01]/ICGEB/Italia | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[741-B4-100]/UCR/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicology Letters; Volumen 257. 2016 | es_ES |
dc.subject | Micrurus lemniscatus | es_ES |
dc.subject | Group I phospholipase A2 | es_ES |
dc.subject | Amino acid sequence | es_ES |
dc.subject | Myotoxicity | es_ES |
dc.subject | Cytotoxicity | es_ES |
dc.subject | Inflammation | es_ES |
dc.title | Lemnitoxin, the major component of Micrurus lemniscatus coral snake venom, is a myotoxic and pro-inflammatory phospholipase A2 | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/j.toxlet.2016.06.005 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.identifier.pmid | 27282409 |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
Microbiología [1171]