Show simple item record

dc.creatorHerrera Vega, María
dc.creatorTattini Jr., Virgilio
dc.creatorPitombo, Ronaldo N. M.
dc.creatorGutiérrez, José María
dc.creatorBorgognoni, Camila
dc.creatorVega Baudrit, José Roberto
dc.creatorSolera, Federico
dc.creatorCerdas Solís, Maykel
dc.creatorSegura Ruiz, Álvaro
dc.creatorVillalta Arrieta, Mauren
dc.creatorVargas Arroyo, Mariángela
dc.creatorLeón Montero, Guillermo
dc.date.accessioned2017-06-16T20:38:23Z
dc.date.available2017-06-16T20:38:23Z
dc.date.issued2014-11
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010114002116es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/30126
dc.description.abstractFreeze-drying is used to improve the long term stability of pharmaceutical proteins. Sugars and polyols have been successfully used in the stabilization of proteins. However, their use in the development of freeze-dried antivenoms has not been documented. In this work, whole IgG snake antivenom, purified from equine plasma, was formulated with different concentrations of sorbitol, sucrose or mannitol. The glass transition temperatures of frozen formulations, determined by Differential Scanning Calorimetry (DSC), ranged between −13.5 °C and −41 °C. In order to evaluate the effectiveness of the different stabilizers, the freeze-dried samples were subjected to an accelerated stability test at 40 ± 2 °C and 75 ± 5% relative humidity. After six months of storage at 40 °C, all the formulations presented the same residual humidity, but significant differences were observed in turbidity, reconstitution time and electrophoretic pattern. Moreover, all formulations, except antivenoms freeze-dried with mannitol, exhibited the same potency for the neutralization of lethal effect of Bothrops asper venom. The 5% (w:v) sucrose formulation exhibited the best stability among the samples tested, while mannitol and sorbitol formulations turned brown. These results suggest that sucrose is a better stabilizer than mannitol and sorbitol in the formulation of freeze-dried antivenoms under the studied conditions.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B2-091]/UCR/Costa Ricaes_ES
dc.description.sponsorshipPrograma Iberoamericano de Ciencia y Tecnología para el Desarrollo/[BIOTOX 212RT0467]/CYTED/Españaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 90. 2014es_ES
dc.subjectAntivenomes_ES
dc.subjectFreeze-dryinges_ES
dc.subjectSorbitoles_ES
dc.subjectMannitoles_ES
dc.subjectSucrosees_ES
dc.subjectStabilityes_ES
dc.titleFreeze-dried snake antivenoms formulated with sorbitol, sucrose or mannitol: Comparison of their stability in an accelerated testes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2014.07.015
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid25091348


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record