Show simple item record

dc.creatorPatiño Llano, Arley Camilo
dc.creatorPereañez, Jaime Andrés
dc.creatorGutiérrez, José María
dc.creatorRucavado Romero, Alexandra
dc.date.accessioned2017-06-09T14:05:21Z
dc.date.available2017-06-09T14:05:21Z
dc.date.issued2013-03-01
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010112008057es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/30077
dc.description.abstractTwo clotting serine proteinases, named Cdc SI and Cdc SII, were isolated and characterized for the first time from Colombian Crotalus durissus cumanensis snake venom. The enzymes were purified using two chromatographic steps: molecular exclusion on Sephacryl S-200 and RP-HPLC on C8 Column. The molecular masses of the proteins, determined by MALDI-TOF mass spectrometry, were 28,561.4 and 28,799.2 Da for Cdc SI and Cdc SII, respectively. The aim of the present study was to evaluate enzymatic, coagulant and toxic properties of the two enzymes. The serine proteinases hydrolyzed specific chromogenic substrate (BaPNA) and exhibited a Michaelis–Menten behavior. Cdc SI had Vmax of 0.038 ± 0.003 nmol/min and KM of 0.034 ± 0.017 mM, while Cdc SII displayed values of Vmax of 0.267 ± 0.011 nmol/min and KM of 0.145 ± 0.023 mM. N-terminal sequences were VIGGDEXNIN and VIGGDICNINEHNFLVALYE for Cdc SI and Cdc SII, respectively. Molecular masses, N-terminal sequences, inhibition assays, and enzymatic profile suggest that Cdc SI and Cdc SII belong to the family of snake venom thrombin-like enzymes. These serine proteinases differed in their clotting activity on human plasma, showing a minimum coagulant dose of 25 μg and 0.571 μg for Cdc SI and Cdc SII, respectively. Enzymes also showed coagulant activity on bovine fibrinogen and degraded chain α of this protein. Toxins lack hemorrhagic and myotoxic activities, but are capable to induce defibrin(ogen)ation, moderate edema, and an increase in vascular permeability. These serine proteinases may contribute indirectly to the local hemorrhage induced by metalloproteinases, by causing blood clotting disturbances, and might also contribute to cardiovascular alterations characteristic of patients envenomed by C. d. cumanensis in Colombia.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B0-506]/UCR/Costa Ricaes_ES
dc.description.sponsorshipConsejo Nacional de Rectores//CONARE/Costa Ricaes_ES
dc.description.sponsorshipComité para el desarrollo de la investigación//CODI/Colombiaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 63. 2013es_ES
dc.subjectThrombin-like enzymees_ES
dc.subjectCoagulant activityes_ES
dc.subjectCrotalus durissus cumanensises_ES
dc.subjectSerine proteinasees_ES
dc.subjectCdc SIes_ES
dc.subjectCdc SIIes_ES
dc.subjectSnake venomes_ES
dc.titleBiochemical and biological characterization of two serine proteinases from Colombian Crotalus durissus cumanensis snake venomes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2012.11.010
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid23178323


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record