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dc.creatorVargas Arroyo, Mariángela
dc.creatorSegura Ruiz, Álvaro
dc.creatorHerrera Vega, María
dc.creatorVillalta Arrieta, Mauren
dc.creatorAngulo Ugalde, Yamileth
dc.creatorGutiérrez, José María
dc.creatorLeón Montero, Guillermo
dc.date.accessioned2017-06-09T13:39:29Z
dc.date.available2017-06-09T13:39:29Z
dc.date.issued2012-08
dc.identifier.citationhttp://onlinelibrary.wiley.com/doi/10.1002/btpr.1565/abstract;jsessionid=B86BC21F2D98CBD795300827E8EF0FA6.f02t04es_ES
dc.identifier.issn1520-6033
dc.identifier.urihttp://hdl.handle.net/10669/30075
dc.description.abstractThe current shortages in human plasma products at global levels justify the development of new, cost effective plasma fractionation methods. We have developed a fractionation process to obtain immunoglobulin G (IgG) and albumin-enriched fractions based on polymer-salt aqueous two phase system (ATPS). A small-scale (0.02 L) ATPS composed of polyethyleneglycol 3350 (PEG), potassium phosphate and sodium chloride, at pH 6.1, was evaluated and subjected to 50-fold scale-up (1 L). Further purification of the fractions was performed using caprylic acid precipitation and ion exchange chromatography. Similar yield and purity were obtained at both small and large scales. IgG precipitated in the PEG rich upper phase at 83% recovery and 2.75-fold purification factor. An 81% pure albumin fraction was obtained in the salt rich bottom phase with a 91% yield. After polishing, IgG was obtained at a recovery of 70% and a purity of 92%. Corresponding values for albumin were 91% and 90%. This IgG and albumin fractionation technology deserves further evaluation as it may represent a potential alternative to conventional plasma fractionation methodses_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B0-113]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceBiotechnology Progress; Volumen 28, Número 4. 2012es_ES
dc.subjectBlood Chemical Analysises_ES
dc.subjectChemical Fractionationes_ES
dc.subjectChromatography, High Pressure Liquides_ES
dc.subjectHumanses_ES
dc.subjectImmunoglobulin Ges_ES
dc.subjectSerum Albumines_ES
dc.titlePurification of IgG and albumin from human plasma by aqueous two phase system fractionationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1002/btpr.1565
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid22619188


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