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dc.creatorVillalta Arrieta, Mauren
dc.creatorPla Ferrer, Davinia
dc.creatorYang, Su Lin
dc.creatorSanz, Libia
dc.creatorSegura Ruiz, Álvaro
dc.creatorVargas Arroyo, Mariángela
dc.creatorChen, Pei Yu
dc.creatorHerrera Vega, María
dc.creatorEstrada Umaña, Ricardo
dc.creatorCheng, Ya Fen
dc.creatorLee, Cheng Dow
dc.creatorCerdas Solís, Maykel
dc.creatorChiang, Jen Ron
dc.creatorAngulo Ugalde, Yamileth
dc.creatorLeón Montero, Guillermo
dc.creatorCalvete Chornet, Juan José
dc.creatorGutiérrez, José María
dc.date.accessioned2017-06-08T17:27:44Z
dc.date.available2017-06-08T17:27:44Z
dc.date.issued2012-10-22
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S187439191200601Xes_ES
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10669/30066
dc.description.abstractThe proteomes of the venoms of the snakes Viridovipera stejnegeri and Protobothrops mucrosquamatus from Taiwan were characterized by N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of in-gel generated tryptic peptides. Proteins belonging to the following toxin classes were identified: metalloproteinase, phospholipase A2 (PLA2), serine proteinase, C-type lectin-like, CRISP, l-amino acid oxidase, disintegrin, and peptides (vasoactive and inhibitors of SVMPs). Nine horses were immunized with a mixture of these venoms. All horses developed a satisfactory immune response against lethality of the venom of V. stejnegeri, whereas only three horses reached the accepted neutralizing potency against the venom of P. mucrosquamatus. Antivenoms were prepared from pools of ‘good responder’ (GR) and ‘poor responder’ (PR) horses and compared by antivenomics and neutralization tests. A similar neutralizing response was observed between the GR and PR antivenoms against the venom of V. stejnegeri, whereas antivenom from PR had a lower neutralizing activity against effects of P. mucrosquamatus venom than antivenom from GR. The low potency of the plasma of some horses against this venom is a consequence of the low immunogenicity of the neurotoxic PLA2 trimucrotoxin. Our results provide clues for innovating the immunization scheme to generate improved antivenoms.es_ES
dc.description.sponsorshipMinisterio de Economía y Competitividad/[BFU2010-17373]/MINECO/Españaes_ES
dc.description.sponsorshipGeneralitat Valenciana/[PROMETEO/2010/005]//Españaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A9-003]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Proteomics; Volumen 75, Número 18. 2012es_ES
dc.subjectViridovipera stejnegeries_ES
dc.subjectProtobothrops mucrosquamatuses_ES
dc.subjectSnake venomicses_ES
dc.subjectAntivenomicses_ES
dc.subjectVenom neutralizationes_ES
dc.subjectImmune responsees_ES
dc.titleSnake venomics and antivenomics of Protobothrops mucrosquamatus and Viridovipera stejnegeri from Taiwan: Keys to understand the variable immune response in horseses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.jprot.2012.08.008
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid22906718


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