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dc.creatorHerrera Vega, María
dc.creatorFernández Ulate, Julián
dc.creatorVargas Arroyo, Mariángela
dc.creatorVillalta Arrieta, Mauren
dc.creatorSegura Ruiz, Álvaro
dc.creatorLeón Montero, Guillermo
dc.creatorAngulo Ugalde, Yamileth
dc.creatorPaiva, Owen K.
dc.creatorMatainaho, Teatulohi
dc.creatorJensen, Simon D.
dc.creatorWinkel, Kenneth D.
dc.creatorCalvete Chornet, Juan José
dc.creatorWilliams, David J.
dc.creatorGutiérrez, José María
dc.date.accessioned2017-03-24T20:35:26Z
dc.date.available2017-03-24T20:35:26Z
dc.date.issued2012-04-03
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S1874391912000231es_ES
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10669/29619
dc.description2082-04 Embargo por política editoriales_ES
dc.description.abstractThe venom proteomes of populations of the highly venomous taipan snake, Oxyuranus scutellatus, from Australia and Papua New Guinea (PNG), were characterized by reverse-phase HPLC fractionation, followed by analysis of chromatographic fractions by SDS-PAGE, N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of tryptic peptides. Proteins belonging to the following seven protein families were identified in the two venoms: phospholipase A2 (PLA2), Kunitz-type inhibitor, metalloproteinase (SVMP), three-finger toxin (3FTx), serine proteinase, cysteine-rich secretory proteins (CRISP), and coagulation factor V-like protein. In addition, C-type lectin/lectin-like protein and venom natriuretic peptide were identified in the venom of specimens from PNG. PLA2s comprised more than 65% of the venoms of these two populations. Antivenoms generated against the venoms of these populations showed a pattern of cross-neutralization, corroborating the immunological kinship of these venoms. Toxicity experiments performed in mice suggest that, at low venom doses, neurotoxicity leading to respiratory paralysis represents the predominant mechanism of prey immobilization and death. However, at high doses, such as those injected in natural bites, intravascular thrombosis due to the action of the prothrombin activator may constitute a potent and very rapid mechanism for killing preyes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A7-611]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A9-506]/UCR/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[BFU2010-17373]//Españaes_ES
dc.description.sponsorshipGeneralitat Valenciana/[PROMETEO/2010/005]//Españaes_ES
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas/2009CR0021/CRUSA-CSIC/Españaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Proteomics; Volumen 75, Número 7. 2012es_ES
dc.subjectTaipanes_ES
dc.subjectOxyuranus scutellatuses_ES
dc.subjectVenomes_ES
dc.subjectTaipoxines_ES
dc.subjectProteomees_ES
dc.subjectAntivenomes_ES
dc.titleComparative proteomic analysis of the venom of the taipan snake, Oxyuranus scutellatus, from Papua New Guinea and Australia: Role of neurotoxic and procoagulant effects in venom toxicityes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.jprot.2012.01.006
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid22266484


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