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A group IIA-secreted phospholipase A2 from snake venom induces lipid body formation in macrophages: the roles of intracellular phospholipases A2 and distinct signaling pathways
dc.creator | Leiguez, Elbio | |
dc.creator | Pavan Zuliani, Juliana | |
dc.creator | Marques Cianciarullo, Aurora | |
dc.creator | Fernandes, Cristina Maria | |
dc.creator | Gutiérrez, José María | |
dc.creator | Teixeira, Catarina de Fátima | |
dc.date.accessioned | 2017-02-27T19:40:57Z | |
dc.date.available | 2017-02-27T19:40:57Z | |
dc.date.issued | 2011-07 | |
dc.identifier.citation | http://www.jleukbio.org/content/90/1/155.long | |
dc.identifier.issn | 0741-5400 | |
dc.identifier.uri | https://hdl.handle.net/10669/29560 | |
dc.description | Versión final embargada hasta 2081-07 por políticas editoriales | es_ES |
dc.description.abstract | We investigated the ability of the sPLA(2), known as MT-III, isolated from the viperid snake Bothrops asper, to induce LB formation in macrophages and the major cellular signaling pathways involved in this process. The effects of MT-III on ADRP localization and expression and macrophage ultrastructure were assessed. Our results showed that this sPLA(2) induced a marked increase in LB numbers in macrophages, induced the recruitment of ADRP in macrophages, and up-regulated ADRP expression. Ultrastructural analysis showed the presence of weakly and strongly osmiophilic LBs in sPLA(2)-stimulated cells. Enlargement of the ER and Golgi cisterns was also observed. Pretreatment of cells with H7 or staurosporine (PKC inhibitors), LY294002 or wortmannin (PI3K inhibitors), SB202190 or PD98059 (p38(MAPK) and ERK1/2 inhibitors, respectively), or Pyr-2 or Bel (cPLA(2) and iPLA(2) inhibitors, respectively) significantly reduced sPLA(2)-induced LB formation. Herbimycin (a PTK inhibitor) and indomethacin or etoricoxib (COX inhibitors) failed to alter sPLA(2)-induced effects. In conclusion, our results show for the first time the ability of a venom sPLA(2) to induce the formation of LBs and the expression of ADRP in macrophages. Venom PLA(2)-induced LB formation is dependent on PKC, PI3K, p38(MAPK), ERK1/2, cPLA(2), and iPLA(2) signaling pathways but not on PTK, COX-1, or COX-2 pathways. Activation of the ER and Golgi complex may play an important role in the formation of LBs induced by this sPLA(2) in macrophages. | es_ES |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo/[06/58334-8]/FAPESP/Brasil | es_ES |
dc.description.sponsorship | Instituto Nacional de Ciencia e Tecnologia em Toxinas//INCTTOX/Brazil | es_ES |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico//CNPq/Brasil | es_ES |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo/[08/58988-3]/FAPESP/Brasil | es_ES |
dc.description.sponsorship | Universidad de Costa Rica//UCR/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Journal of Leukocyte Biology; Volumen 90, Número 1. 2011 | es_ES |
dc.subject | Animals | es_ES |
dc.subject | Blotting, Western | es_ES |
dc.subject | Bothrops | es_ES |
dc.subject | Crotalid Venoms | es_ES |
dc.subject | Endoplasmic Reticulum | es_ES |
dc.subject | Golgi Apparatus | es_ES |
dc.subject | Group II Phospholipases A2 | es_ES |
dc.subject | Immunohistochemistry | es_ES |
dc.subject | Inclusion Bodies | es_ES |
dc.subject | Lipids | es_ES |
dc.subject | Macrophages | es_ES |
dc.subject | Male | es_ES |
dc.subject | Mice | es_ES |
dc.subject | Microscopy, Electron, Transmission | es_ES |
dc.subject | Signal Transduction | es_ES |
dc.title | A group IIA-secreted phospholipase A2 from snake venom induces lipid body formation in macrophages: the roles of intracellular phospholipases A2 and distinct signaling pathways | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1189/jlb.0510263 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.identifier.pmid | 21478270 |
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Microbiología [1171]