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dc.creatorEscalante Muñoz, Teresa
dc.creatorNúñez, Javier
dc.creatorMoura Da Silva, Ana M.
dc.creatorRucavado Romero, Alexandra
dc.creatorTheakston, R. David G.
dc.creatorGutiérrez, José María
dc.date.accessioned2017-02-07T15:44:29Z
dc.date.available2017-02-07T15:44:29Z
dc.date.issued2003-11-15
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041008X03003375
dc.identifier.issn0041-008X
dc.identifier.urihttps://hdl.handle.net/10669/29506
dc.description.abstractJararhagin is the most important hemorrhagic component in the venom of the snake Bothrops jararaca, a species of medical importance in South America. It is a P-III zinc-dependent metalloproteinase comprising catalytic, disintegrin-like, and cysteine-rich domains. Jararhagin injected intravenously into mice induced rapid and prominent bleeding in the lungs, whereas other organs were devoid of overt hemorrhagic manifestations. This action depends on the proteolytic activity of jararhagin, since it was abrogated by the synthetic inhibitor batimastat. There were conspicuous ultrastructural alterations in cells at the alveolo-capillary unit, i.e., capillary endothelial cells and type I pneumocytes, with a characteristic pattern of “regional alveolar damage” associated with extravasation. These pathological effects were observed under conditions in which the whole blood clotting time, bleeding time, and fibrinogen levels were not affected. 125I-labeled jararhagin is concentrated mainly in liver and kidneys after iv injection, with little radioactivity observed in the lungs, thereby indicating that the predominance of pulmonary microvascular damage is not due to a preferential concentration of this enzyme in the lungs. Despite the fact that jararhagin is complexed by plasma proteins after iv injection, its hemorrhagic activity was not inhibited by the plasma proteinase inhibitor α2-macroglobulin, and was only partially reduced by normal mouse serum, suggesting that resistance to inhibition may contribute to its ability to cause pulmonary hemorrhage.es_ES
dc.description.sponsorshipWellcome Trust/[062043]//Inglaterraes_ES
dc.description.sponsorshipInternational Foundation for Science/[F-2707-2]/IFS/Sueciaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A1-504]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A2-036]/UCR/Costa Ricaes_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo//FAPESP/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceToxicology and Applied Pharmacology; Volumen 193, Número 1. 2003es_ES
dc.subjectHemorrhagees_ES
dc.subjectMetalloproteinasees_ES
dc.subjectJararhagines_ES
dc.subjectα2-Macroglobulines_ES
dc.subjectAlveolar Damagees_ES
dc.subjectSnake venomes_ES
dc.titlePulmonary hemorrhage induced by jararhagin, a metalloproteinase from Bothrops jararaca snake venomes_ES
dc.typeartículo original
dc.identifier.doi10.1016/S0041-008X(03)00337-5
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-A1-504
dc.identifier.codproyecto741-A2-036
dc.identifier.pmid14613713


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