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dc.creatorSaldarriaga Córdoba, Mónica Marı́a
dc.creatorOtero Patiño, Rafael
dc.creatorNúñez Rangel, Vitelbina
dc.creatorToro, María Fabiola
dc.creatorDı́az, Abel
dc.creatorGutiérrez, José María
dc.date.accessioned2017-02-06T21:45:55Z
dc.date.available2017-02-06T21:45:55Z
dc.date.issued2003-09
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010103001715es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29503
dc.description.abstractThe lancehead snakes Bothrops asper and Bothrops atrox inflict 70–90% of the 3000 bites reported every year in Colombia. In this work, the venoms of B. atrox from Meta (Villavicencio, 33 specimens) and B. asper from Antioquia (San Carlos, 45 specimens), all of them born in captivity, were obtained at different ages (0–6 months; 1, 2 and 3-years old) and compared in terms of their pharmacological and immunochemical characteristics. A conspicuous ontogenetic variability was observed in venom samples from both species. Venoms from newborn and juvenile specimens showed higher lethal, hemorrhagic, edema-forming and coagulant activities, whereas venoms from 3-year old specimens showed higher indirect hemolytic, i.e. phospholipase A2 activity, being more significant in the case of B. asper. SDS-polyacrylamide gel electrophoresis of whole venom for both species evidenced a predominance of high mol. mass bands in the venoms from specimens of <1 year of age, with a change towards bands having lower mol. mass as snakes aged. Gel filtration chromatography showed five peaks in the venoms of B. asper of <6 months and in those from 3-year old specimens. Venom of adult specimens showed a higher number of peaks with indirect hemolytic activity than venom of newborn specimens. Polyvalent antivenom produced in Costa Rica recognized all the bands of both venoms from specimens at all ages tested, when assayed by Western blotting.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A1-027]/UCR/Costa Ricaes_ES
dc.description.sponsorshipInstituto Colombiano para el Desarrollo de la Ciencia y la Tecnología Francisco José de Caldas//COLCIENCIAS/Colombiaes_ES
dc.description.sponsorshipBanco Interamericano de Desarrollo//BID/es_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 42, Número 4, 2003es_ES
dc.subjectBothrops Atroxes_ES
dc.subjectBothrops Asperes_ES
dc.subjectColombiaes_ES
dc.subjectVenom Variabilityes_ES
dc.titleOntogenetic variability of Bothrops atrox and Bothrops asper snake venoms from Colombiaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/S0041-0101(03)00171-5
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-A1-027
dc.identifier.pmid14505941


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