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dc.creatorSquaiella Baptistão, Carla Cristinaes_ES
dc.creatorMarcelino, José Robertoes_ES
dc.creatorRibeiro da Cunha, Luiz Eduardoes_ES
dc.creatorGutiérrez, José Maríaes_ES
dc.creatorTambourgi, Denise V.es_ES
dc.date.accessioned2017-01-16T19:53:56Zes_ES
dc.date.available2017-01-16T19:53:56Zes_ES
dc.date.issued2014es_ES
dc.identifier.citationhttp://www.ajtmh.org/content/90/3/574es_ES
dc.identifier.issn1476-1645es_ES
dc.identifier.urihttp://hdl.handle.net/10669/29427es_ES
dc.description2094-01 Embargo por política editoriales_ES
dc.description.abstractEnvenomation by poisonous animals is a neglected condition according to the World Health Organization (WHO). Antivenoms are included in the WHO Essential Medicines List. It has been assumed that immunoglobulin G (IgG) antivenoms could activate the complement system through Fc and induce early adverse reactions (EARs). However, data in the literature indicate that F(ab')2 fragments can also activate the complement system. Herein, we show that several batches of IgG and F(ab')2 antivenoms from the Butantan, Vital Brazil, and Clodomiro Picado Institutes activated the complement classical pathway and induced the production of C3a; however, only those antivenoms from Clodomiro Picado generated C5a. Different protein profiles (IgG heavy chain, protein contaminants, and aggregates) were observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analyses. Our results show that various antivenoms from different producers are able to activate the classical pathway of the complement system and generate anaphylatoxins, and these findings suggest that factors, such as composition, contaminant proteins, and aggregates, may influence the anticomplementary activity of antivenoms in vitro. Therefore, there is a need to further improve antivenom production methods to reduce their anticomplementary activity and potential to cause EARs.es_ES
dc.description.sponsorshipSao Paulo Research Foundation/[2011/51869-1]/FAPESP/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceThe American Journal of Tropical Medicine and Hygiene; Volumen 90, Número 3. 2014es_ES
dc.subjectAnaphylatoxinses_ES
dc.subjectAnimalses_ES
dc.subjectAntiveninses_ES
dc.subjectBlotting, Westernes_ES
dc.subjectComplement Activationes_ES
dc.subjectComplement C3aes_ES
dc.subjectComplement C5aes_ES
dc.subjectComplement Pathway, Classicales_ES
dc.subjectCrotalid Venomses_ES
dc.subjectElectrophoresis, Polyacrylamide Geles_ES
dc.subjectHorseses_ES
dc.subjectHumanses_ES
dc.subjectImmunoglobulin Fab Fragmentses_ES
dc.subjectImmunoglobulin Ges_ES
dc.subjectImmunologic Factorses_ES
dc.subjectNeutralization Testses_ES
dc.subjectRabbitses_ES
dc.subjectScorpion Venomses_ES
dc.subjectSheepes_ES
dc.titleAnticomplementary Activity of Horse IgG and F(ab’)2 Antivenomses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.4269/ajtmh.13-0591es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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