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dc.creatorZuliani, Juliana Pavanes_ES
dc.creatorGutiérrez, José Maríaes_ES
dc.creatorCasais e Silva, Luciana Lyraes_ES
dc.creatorCoccuzzo Sampaio, Sandraes_ES
dc.creatorLomonte, Brunoes_ES
dc.creatorTeixeira, Catarina de Fátimaes_ES
dc.date.accessioned2017-01-09T20:23:49Zes_ES
dc.date.available2017-01-09T20:23:49Zes_ES
dc.date.issued2005-10es_ES
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010105002266es_ES
dc.identifier.issn0041-0101es_ES
dc.identifier.urihttp://hdl.handle.net/10669/29416es_ES
dc.description.abstractThe in vitro effects of myotoxin III (MT-III), an Asp-49 catalytically-active phospholipase A(2), and myotoxin II (MT-II), a catalytically-inactive Lys-49 variant, isolated from Bothrops asper snake venom, on phagocytosis and production of hydrogen peroxide (H(2)O(2)) by thioglycollate-elicited macrophages were investigated. MT-II and MT-III were cytotoxic to mouse peritoneal macrophages at concentrations higher than 25 microg/ml. At non-cytotoxic concentrations, MT-II stimulated Fcgamma, complement, mannose and beta-glucan receptors-mediated phagocytosis, whereas MT-III stimulated only the mannose and beta-glucan receptors-mediated phagocytosis. Moreover, both myotoxins induced the release of H(2)O(2) by thioglycollate-elicited macrophages, MT-III being the most potent stimulator. MT-II induced the release of H(2)O(2) only at a concentration of 3.2 microg/ml (130% increment) while MT-III induced this effect at all concentrations tested (0.5-2.5 microg/ml; average of 206% increment). It is concluded that, at non-cytotoxic concentrations, MT-II and MT-III activate defense mechanisms in macrophages up regulating phagocytosis, mainly via mannose and beta-glucan receptors, and the respiratory burst.es_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo/[02/13863-2]/FAPESP/Brasiles_ES
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico/[301199/91-4]/CNPq/Brasiles_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo/[98/1565-7]/FAPESP/Brasiles_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo/[02/01009-7]/FAPESP/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 46, Número 5. 2005es_ES
dc.subjectVenom Myotoxic PLA2es_ES
dc.subjectMacrophageses_ES
dc.subjectPhagocytosises_ES
dc.subjectHydrogen peroxidees_ES
dc.subjectInflammationes_ES
dc.titleActivation of cellular functions in macrophages by venom secretory Asp-49 and Lys-49 phospholipases A2es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2005.06.017es_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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