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Calcium plays a key role in the effects induced by a snake venom Lys49 phospholipase A2 homologue on a lymphoblastoid cell line
dc.creator | Mora Rodríguez, Rodrigo Antonio | |
dc.creator | Maldonado, Alexis | |
dc.creator | Valverde Rojas, Berta E. | |
dc.creator | Gutiérrez, José María | |
dc.date.accessioned | 2016-12-15T16:01:34Z | |
dc.date.available | 2016-12-15T16:01:34Z | |
dc.date.issued | 2006-01 | |
dc.identifier.issn | 0041-0101 | |
dc.identifier.uri | https://hdl.handle.net/10669/29406 | |
dc.description.abstract | A catalytically-inactive Lys49 phospholipase A2 homologue from the venom of the snake Bothrops asper induces diverse effects (necrosis, apoptosis and proliferation) in a lymphoblastoid cell line, depending on the toxin concentration. The increments in cytosolic Ca2+ levels induced by this toxin in this cell line were assessed. At high toxin concentration (100 microg/mL) the toxin induces drastic disruption of the plasma membrane, associated with a prominent Ca2+ influx and necrosis. Previous incubation of the cells with the chelating agent EGTA or with ruthenium red, an inhibitor of the uniporter mitochondrial Ca2+ transport, greatly reduced necrosis. At a toxin concentration of 12.5 microg/mL, apoptosis is the predominant response, being associated with lower increments in cytosolic Ca2+. This effect was inhibited by preincubation with ruthenium red and the cytosolic Ca2+ chelator BAPTA-AM. The proliferative response, which occurs at a low toxin concentration (0.5 microg/mL), is associated with a small and oscillatory increment in cytosolic Ca2+. It was inhibited by EGTA, ruthenium red and BAPTA-AM, by inhibitors of the endoplasmic reticulum Ca2+ -ATPase (SERCA) and by blockade of the ryanodine receptor. It is concluded that necrosis and apoptosis induced by this toxin are associated with increments in cytosolic Ca2+ levels following plasma membrane perturbation, together with the involvement of mitochondria. The cellular proliferative response depends on a limited Ca2+ influx through the plasma membrane, being associated with a concerted functional unit constituted by SERCA, the ryanodine receptor and mitochondria, which regulate the observed oscillations in cytosolic Ca2+ concentration. | es_ES |
dc.description.sponsorship | Universidad de Costa Rica//UCR/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicon; Volumen 47, Número 1. 2006 | es_ES |
dc.subject | Ca2 + Homeostasis | es_ES |
dc.subject | Lys49 Phospholipase A2 | es_ES |
dc.subject | Mitochondria | es_ES |
dc.subject | SERCA | es_ES |
dc.subject | Plasma Membrane | es_ES |
dc.subject | Necrosis | es_ES |
dc.subject | Apoptosis | es_ES |
dc.subject | Snake venom | es_ES |
dc.title | Calcium plays a key role in the effects induced by a snake venom Lys49 phospholipase A2 homologue on a lymphoblastoid cell line | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/j.toxicon.2005.10.001 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
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Microbiología [1170]