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dc.creatorAlape Girón, Albertoes_ES
dc.creatorStiles, Bradley G.es_ES
dc.creatorGutiérrez, José Maríaes_ES
dc.date.accessioned2016-12-08T18:15:37Zes_ES
dc.date.available2016-12-08T18:15:37Zes_ES
dc.date.issued1996-03es_ES
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/0041010195001263es_ES
dc.identifier.issn0041-0101es_ES
dc.identifier.urihttp://hdl.handle.net/10669/29371es_ES
dc.description.abstractAn ELISA based, non-radioactive acetylcholine receptor (AchR) binding assay was used to detect the alpha-neurotoxins present in Micrurus nigrocinctus nigrocinctus venom. Sera from horses hyperimmunized against M. nigrocinctus venom contain antibodies which inhibit the binding of M. n. nigrocinctus alpha-neurotoxins to AchR and reverse the binding of toxins already complexed with the receptor. This result supports the importance of using antivenom therapeutically in M. n. nigrocinctus envenomations even after the onset of neurological symptoms. M. nigrocinctus antivenoms cross-reacted in an ELISA with several elapid alpha-neurotoxins and inhibited the binding of Bungarus multicinctus alpha-bungarotoxin and Naja naja oxiana neurotoxin II to AchR in vitro, suggesting the presence of short-chain and long-chain alpha-neurotoxins in M. nigrocinctus venom. In vivo neutralization experiments with M. nigrocinctus antivenom demonstrate that M. nigrocinctus venom contains short-chain alpha-neurotoxin(s) which share common neutralizing epitope(s) with Naja naja oxiana neurotoxin II.es_ES
dc.description.sponsorshipSwedish Agency for Research Cooperation with Developing Countries//SAREC/Sueciaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-93-301]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 34, Número 3, 1996es_ES
dc.subjectAnimalses_ES
dc.subjectAntibodieses_ES
dc.subjectAntiveninses_ES
dc.subjectBinding, Competitivees_ES
dc.subjectCross Reactionses_ES
dc.subjectDose-Response Relationship, Druges_ES
dc.subjectElapid Venomses_ES
dc.subjectElapidaees_ES
dc.subjectEnzyme-Linked Immunosorbent Assayes_ES
dc.subjectGuinea Pigses_ES
dc.subjectHorseses_ES
dc.subjectImmune Seraes_ES
dc.subjectImmunizationes_ES
dc.subjectMicees_ES
dc.subjectNeurotoxinses_ES
dc.subjectNeutralization Testses_ES
dc.subjectReceptors, Cholinergices_ES
dc.subjectSnake venomes_ES
dc.titleAntibody-mediated neutralization and binding-reversal studies on α-neurotoxins from micrurus nigrocinctus nigrocinctus (coral snake) venomes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/0041-0101(95)00126-3es_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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