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dc.creatorGutiérrez, José María
dc.creatorSanz, Libia
dc.creatorFlores Díaz, Marietta
dc.creatorFigueroa, Lucía
dc.creatorMadrigal Villalobos, Marvin
dc.creatorHerrera Vega, María
dc.creatorVillalta Arrieta, Mauren
dc.creatorLeón Montero, Guillermo
dc.creatorEstrada Umaña, Ricardo
dc.creatorBorges, Adolfo
dc.creatorAlape Girón, Alberto
dc.creatorCalvete Chornet, Juan José
dc.date.accessioned2016-12-06T16:33:42Z
dc.date.available2016-12-06T16:33:42Z
dc.date.issued2010-10-21
dc.identifier.citationhttp://pubs.acs.org/doi/abs/10.1021/pr9009518es_ES
dc.identifier.issn1535-3907
dc.identifier.urihttp://hdl.handle.net/10669/29348
dc.description.abstractIntraspecific snake venom variations have implications in the preparation of venom pools for the generation of antivenoms. The impact of such variation in the cross-reactivity of antivenoms against Bothrops asper venom was assessed by comparing two commercial and four experimental antivenoms. All antivenoms showed similar immunorecognition pattern toward the venoms from adult and neonate specimens. They completely immunodepleted most P−III snake venom metalloproteinases (SVMPs), l-amino acid oxidases, serine proteinases, DC fragments, cysteine-rich secretory proteins (CRISPs), and C-type lectin-like proteins, and partially immunodepleted medium-sized disintegrins, phospholipases A2 (PLA2s), some serine proteinases, and P−I SVMPs. Although all antivenoms abrogated the lethal, hemorrhagic, coagulant, proteinase, and PLA2 venoms activities, monospecific experimental antivenoms were more effective than the polyspecific experimental antivenom. In addition, the commercial antivenoms, produced in horses subjected to repeated immunization cycles, showed higher neutralization than experimental polyspecific antivenom, produced by a single round of immunization. Overall, a conspicuous pattern of cross-neutralization was evident for all effects by all antivenoms, and monospecific antivenoms raised against venom from the Caribbean population were effective against venom from the Pacific population, indicating that geographic variations in venom proteomes of B. asper from Costa Rica do not result in overt variations in immunological cross-reactivity between antivenoms.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A7-611]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A9-003]/UCR/Costa Ricaes_ES
dc.description.sponsorshipCiencia y Tecnología para el Desarrollo/206AC028/CYTED/Españaes_ES
dc.description.sponsorshipMinisterio de Educación y Ciencia/[BFU2007-61563]/MECD/Españaes_ES
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas/2007CR0004/CRUSA-CSIC/Españaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal Proteome Research; Volumen 9 ,Número 1. 2010es_ES
dc.subjectAntivenomes_ES
dc.subjectAntivenomicses_ES
dc.subjectBothrops asperes_ES
dc.subjectPhospholipases A2es_ES
dc.subjectSnake venom metalloproteinasees_ES
dc.subjectSnake venom proteomees_ES
dc.subjectVenom neutralization assayses_ES
dc.subjectVenomicses_ES
dc.subjectSnake venomes_ES
dc.titleImpact of Regional Variation in Bothrops asper Snake Venom on the Design of Antivenoms: Integrating Antivenomics and Neutralization Approacheses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1021/pr9009518
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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