Impact of Regional Variation in Bothrops asper Snake Venom on the Design of Antivenoms: Integrating Antivenomics and Neutralization Approaches
Gutiérrez, José María
Flores Díaz, Marietta
Madrigal Villalobos, Marvin
Herrera Vega, María
León Montero, Guillermo
Estrada Umaña, Ricardo
Alape Girón, Alberto
Calvete, Juan J.
MetadatosMostrar el registro completo del ítem
Intraspecific snake venom variations have implications in the preparation of venom pools for the generation of antivenoms. The impact of such variation in the cross-reactivity of antivenoms against Bothrops asper venom was assessed by comparing two commercial and four experimental antivenoms. All antivenoms showed similar immunorecognition pattern toward the venoms from adult and neonate specimens. They completely immunodepleted most P−III snake venom metalloproteinases (SVMPs), l-amino acid oxidases, serine proteinases, DC fragments, cysteine-rich secretory proteins (CRISPs), and C-type lectin-like proteins, and partially immunodepleted medium-sized disintegrins, phospholipases A2 (PLA2s), some serine proteinases, and P−I SVMPs. Although all antivenoms abrogated the lethal, hemorrhagic, coagulant, proteinase, and PLA2 venoms activities, monospecific experimental antivenoms were more effective than the polyspecific experimental antivenom. In addition, the commercial antivenoms, produced in horses subjected to repeated immunization cycles, showed higher neutralization than experimental polyspecific antivenom, produced by a single round of immunization. Overall, a conspicuous pattern of cross-neutralization was evident for all effects by all antivenoms, and monospecific antivenoms raised against venom from the Caribbean population were effective against venom from the Pacific population, indicating that geographic variations in venom proteomes of B. asper from Costa Rica do not result in overt variations in immunological cross-reactivity between antivenoms.
Enlace externo al ítem10.1021/pr9009518
- Microbiología 
Mostrando ítems relacionados por Título, autor o materia.
Comparative study of the cytolytic activity of myotoxic phospholipases A2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells in vitro Lomonte, Bruno; Angulo, Yamileth; Rufini, Stefano; Cho, Wonhwa; Giglio, José Roberto; Ohno, Motoroni; Daniele, José Julían; Geoghegan, Patricia; Gutiérrez, José María (1999-01)A rapid in vitro cytolytic effect of some myotoxic phospholipases A2 (PLA2s) isolated from the venoms of Viperidae snakes has been previously described. This study was undertaken to investigate if cytolytic activity is a ...
Integrated “omics” profiling indicates that miRNAs are modulators of the ontogenetic venom composition shift in the Central American rattlesnake, Crotalus simus simus Durban, Jordi; Pérez, Alicia; Sanz, Libia; Gómez Argüello, Aarón; Bonilla Murillo, Fabián; Rodríguez Arguedas, Santos; Chacón Gutiérrez, Danilo; Sasa, Mahmood; Angulo, Yamileth; Gutiérrez, José María; Calvete, Juan J. (2013)Background Understanding the processes that drive the evolution of snake venom is a topic of great research interest in molecular and evolutionary toxinology. Recent studies suggest that ontogenetic changes in venom ...
Combined venom gland cDNA sequencing and venomics of the New Guinea small-eyed snake, Micropechis ikaheka Paiva, Owen K.; Pla, Davinia; Wright, Christine E.; Beutler, Markus; Sanz, Libia; Gutiérrez, José María; Williams, David J.; Calvete, Juan J. (2014-10-14)The venom arsenal of the New Guinea small-eyed snake, Micropechis ikaheka, was investigated by a joint cDNA sequencing and venomics approach. Twenty-seven full-length DNA sequences encoding novel venom proteins were recovered ...