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Isolation and characterization of synergistic hemorrhagins from the venom of the snake Bothrops asper
dc.creator | Borkow, Gadi | |
dc.creator | Gutiérrez, José María | |
dc.creator | Ovadia, Michael | |
dc.date.accessioned | 2016-11-11T14:32:36Z | |
dc.date.available | 2016-11-11T14:32:36Z | |
dc.date.issued | 1993-09 | |
dc.identifier.issn | 0041-0101 | |
dc.identifier.uri | https://hdl.handle.net/10669/29227 | |
dc.description.abstract | Three hemorrhagic factors (BaH1, BH2 and BH3) were isolated from the venom of Bothrops asper by gel filtration on Sephacryl S-200, DEAE-Sepharose chromatography, metal chelate affinity chromatography and hydrophobic interaction chromatography. They contain 55% of the total hemorrhagic activity of the whole venom when they are mixed, but lose almost half of the activity if they are separated, indicating a synergism between the three. The main hemorrhagin is BaH1 (Bothrops asper hemorrhagin 1); the other two are weak hemorrhagins but contribute to the synergism. They are acidic proteins with a pI of 4.5, 5.2 and 5; their mol. wt is 64,000, 26,000 and 55,000 respectively. The minimal hemorrhagic dose (MHD) of BaH1, BH2 and BH3 is 0.18, 2 and 1.6 micrograms, with a specific activity 55, 5 and 6.25 higher than that of the whole venom. The hemorrhagic activity of all three factors was inhibited by EDTA and ortho-phenathroline, indicating that the hemorrhagic activity is metal dependent. Phosphoramidon, soybean trypsin inhibitor, PMSF, pepstatin and aprotinin did not affect the hemorrhagic activity of the isolated factors. | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicon; Volumen 31, Número 9, 1993 | es_ES |
dc.subject | Aminosalicylic Acid | es_ES |
dc.subject | Ammonium Sulfate | es_ES |
dc.subject | Animals | es_ES |
dc.subject | Antibody Specificity | es_ES |
dc.subject | Antivenins | es_ES |
dc.subject | Chromatography, Gel | es_ES |
dc.subject | Chromatography, Ion Exchange | es_ES |
dc.subject | Cross Reactions | es_ES |
dc.subject | Crotalid Venoms | es_ES |
dc.subject | Drug Synergism | es_ES |
dc.subject | Endopeptidases | es_ES |
dc.subject | Enzyme Inhibitors | es_ES |
dc.subject | Hemorrhage | es_ES |
dc.subject | Hydrogen-Ion Concentration | es_ES |
dc.subject | Immunodiffusion | es_ES |
dc.subject | Immunoelectrophoresis | es_ES |
dc.subject | Isoelectric Focusing | es_ES |
dc.subject | Mice | es_ES |
dc.subject | Molecular Weight | es_ES |
dc.title | Isolation and characterization of synergistic hemorrhagins from the venom of the snake Bothrops asper | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/0041-0101(93)90129-7 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
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