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dc.creatorDíaz Oreiro, Cecilia
dc.creatorGutiérrez, José María
dc.creatorLomonte, Bruno
dc.creatorGené, José Antonio
dc.date.accessioned2016-11-10T14:15:44Z
dc.date.available2016-11-10T14:15:44Z
dc.date.issued1991-12
dc.identifier.issn0005-2736
dc.identifier.urihttp://hdl.handle.net/10669/29171
dc.description.abstractThe effect of four myotoxins isolated from Bothrops snake venoms on the release of peroxidase trapped in large multilamellar liposomes was studied and correlated to their phospholipase A2, myotoxic and anticoagulant activities. The four myotoxins affected negatively-charged liposomes in a dose-dependent way, having no effect on positively-charged liposomes. Conditions that inhibited phospholipase A2 activity, i.e., substitution of calcium by EDTA, reduced liposome-disrupting activity of Bothrops asper myotoxin I and Bothrops atrox myotoxin, both of which have high phospholipase A2 activity, but did not affect the action of B. asper myotoxin II and Bothrops moojeni myotoxin II, which have extremely low phospholipase A2 activity. However, all myotoxins disrupted to some extent negatively-charged liposomes under conditions where phospholipase A2 activity was abolished. Since these toxins behave as amphiphilic proteins in charge-shift electrophoresis, it is suggested that membrane-disorganization is at least partially due to a non-enzymatic penetration and alteration of bilayers. There was no strict correlation between liposome-disrupting activity and myotoxicity in vivo. Thus, although both effects probably depend on the toxins' ability to disturb membranes, it is likely that variation in complexity between skeletal muscle plasma membrane and liposome bilayers are the basis for this difference. The anticoagulant effect seems to depend on the ability of the toxins to enzymatically degrade phospholipids, since only B. asper myotoxin I and B. atrox myotoxin prolonged the plasma recalcification time.es_ES
dc.language.isoen_USes_ES
dc.sourceBiochimica et Biophysica Acta (BBA) - Biomembranes. Volumen 1070, Número 2, 1991es_ES
dc.subjectAnimalses_ES
dc.subjectAnticoagulantses_ES
dc.subjectCalciumes_ES
dc.subjectCreatine Kinasees_ES
dc.subjectCrotalid Venomses_ES
dc.subjectEgtazic Acides_ES
dc.subjectHorseradish Peroxidasees_ES
dc.subjectLiposomeses_ES
dc.subjectMicees_ES
dc.subjectMuscleses_ES
dc.subjectPhosphatidylcholineses_ES
dc.subjectPhospholipases Aes_ES
dc.subjectPhospholipases A2es_ES
dc.subjectSheepes_ES
dc.subjectSnake venomes_ES
dc.titleThe effect of myotoxins isolated from Bothrops snake venoms on multilamellar liposomes: relationship to phospholipase A2, anticoagulant and myotoxic activitieses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES


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