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dc.creatorSittenfeld Appel, Ana
dc.creatorRaventós Vorst, Henriette
dc.creatorCruz, R.
dc.creatorGutiérrez, José María
dc.date.accessioned2016-11-10T14:00:34Z
dc.date.available2016-11-10T14:00:34Z
dc.date.issued1991
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/004101019190194Ves_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29170
dc.description.abstractDNase activity of Costa Rican crotaline snake venoms from the genera Bothrops, Crotalus and Lachesis was quantified by an enzymodiffusion method on agarose/DNA gels containing ethidium bromide. The reaction is detected as a ring lacking fluorescence when gels are visualized under u.v. light. Electrophoresis of non-fluorescent areas demonstrated DNA degradation. All of the venoms had DNase activity, B. schlegelii being most active. Venoms from B. schlegelii and B. asper induced an inner hyper-fluorescent ring in addition to the external non-fluorescent ring, probably caused by the formation of complexes between DNA and highly basic proteins present in these venoms. In order to study the number of electrophoretic DNase variants, venoms were separated by analytical isoelectric focusing on polyacrylamide minigels, proteins were transferred to nitrocellulose paper and the paper was placed over an agarose gel containing DNA. Then the agarose gel was stained with ethidium bromide and the bands of DNase activity were visualized under u.v. light. All the venoms tested, as well as commercial DNase showed several bands with DNase activity. The majority of venom DNase variants have basic pIs although bands with acidic pIs were also present in B. godmani and L. muta venoms. No major differences in the DNase electrophoretic pattern were observed between individual venoms of adult B. asper specimens nor between lyophilized and frozen venoms.es_ES
dc.language.isoen_USes_ES
dc.sourceToxicon Volumen 29, Número 10, 1991es_ES
dc.subjectCrotalid Venomses_ES
dc.subjectDeoxyribonucleaseses_ES
dc.subjectDiffusiones_ES
dc.subjectElectrophoresises_ES
dc.subjectFreeze Dryinges_ES
dc.subjectFreezinges_ES
dc.subjectImmunoblottinges_ES
dc.subjectIsoelectric Focusinges_ES
dc.subjectProteinses_ES
dc.subjectSpecies Specificityes_ES
dc.titleDNase activity in Costa Rican crotaline snake venoms: quantification of activity and identification of electrophoretic variants.es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/0041-0101(91)90194-V
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES


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