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Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela

dc.creatorMarquina, María Eugenia
dc.creatorGonzález, Néstor Enrique
dc.creatorCastro, Yulimar
dc.date2010-08-10
dc.date.accessioned2016-05-03T15:35:50Z
dc.date.available2016-05-03T15:35:50Z
dc.identifierhttp://revistas.ucr.ac.cr/index.php/rbt/article/view/3375
dc.identifier10.15517/rbt.v0i0.3375
dc.identifier.urihttps://hdl.handle.net/10669/27612
dc.descriptionRhizobial taxonomy and systematics have progressed substantially, nevertheless, few studies have been developed on venezuelan species. This study evaluated the phenotypic and genetic variation between 12 venezuelan indigenous rhizobial isolates and 10 international referential strains, by phenotypical traits and DNA molecular markers. In this regard, a PCR-RFLP of the 16S rDNA gene, the presence of large plasmids, metabolic assays in solid media, salinity resistance, pH and temperature growth conditions, and intrinsic antibiotic resistance were assayed. In reference to the phenotypic attributes, we recognized three main groups: A group I, which comprised all the strains metabolizing between 67.5%-90% of the C and N sources. They were also acid-tolerant, as well as acid producers, capable of growing at 40°C and in high salinity conditions (2-2.5% NaCl). With regard to the antibiotic sensitivity, this group was susceptible to a 30% of the antibiotic assayed. Strains belonging to Group II exhibited a lower salt tolerance (0.1-1.5%NaCl), as well as a lower acid tolerance, since they grew well at pH values equal or higher than 5.0. This group appeared to be resistant to all of the antibiotics assayed and only metabolized between 52.5%-82.5% of the C and N sources. Group III was represented by a single bacterial strain: it has a extremely low salt tolerance (0.1% NaCl). This strain grew at a pH equal or higher than 5.6, was susceptible to 50% of the antibiotics assayed and metabolized 72% of the C and N sources. On the basis of a PCR- RFLP of the 16S rDNA, three groups were also obtained. Members of the group A showed a close resemblance to Rhizobium tropici CIAT 899 and Sinorhizobium americanum CFN-EI 156, while Group B was closely related to Bradyrhizobium spp. Group C, was also represented by only one isolate. The Trebol isolate, was the only one strain able to form nodules and does not appear to be related to any of the referential rhizobial strains, suggesting a possible symbiotic horizontal gene transfer. Finally, in this work, there are evidences of a genetic diversity in the venezuelan rhizobial strains. A different geographical origin is perhaps an important factor affecting the diversity of the indigenous rhizobia in this study.es-ES
dc.formatapplication/pdf
dc.languagespa
dc.publisherUniversidad de Costa Ricaen-US
dc.rightsCopyright (c) 2014 International Journal of Tropical Biology and Conservationen-US
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 59 (3) September 2011en-US
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 59 (3) September 2011es-ES
dc.sourceRevista Biología Tropical; Vol. 59 (3) September 2011pt-PT
dc.source2215-2075
dc.source0034-7744
dc.source10.15517/rbt.v0i0
dc.titleCaracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuelaes-ES
dc.typeartículo original
dc.coverageCRCes-ES


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