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Presence and significance of Bacillus thuringiensis Cry proteins associated with the Andean weevil Premnotrypes vorax (Coleoptera: Curculionidae)

dc.creatorLópez Pazos, Silvio Alejandro
dc.creatorMartínez, John Wilson
dc.creatorCastillo, Adriana Ximena
dc.creatorCerón Salamanca, Jairo Alonso
dc.date2008-10-15
dc.date.accessioned2016-05-03T15:27:59Z
dc.date.available2016-05-03T15:27:59Z
dc.identifierhttp://revistas.ucr.ac.cr/index.php/rbt/article/view/5460
dc.identifier10.15517/rbt.v57i4.5460
dc.identifier.urihttps://hdl.handle.net/10669/26296
dc.descriptionThe Andean weevil Premnotrypes vorax represents an important cause of damage to Colombian potato crops. Due to the impact of this plague on the economy of the country, we searched for new alternatives for its biological control, based on the entomopathogenic bacteria Bacillus thuringiensis. A total of 300 B. thuringiensis strains obtained from potato plantations infested with P. vorax were analyzed through crystal morphology, SDS-PAGE, PCR and bioassays. We used site-directed mutagenesis to modify the Cry3Aa protein. Most of the B. thuringiensis isolates had a bipyramidal crystal morphology. SDS-PAGE analyses had seven strains groups with ?-endotoxins from 35 to 135 kDa. The genes cry 2 and cry 1 were significantly more frequent in the P. vorax habitat (PCR analyses). Three mutant toxins, 1 (D354E), 2 (R345A, ?Y350, ?Y351), and 3 (Q482A, S484A, R485A), were analyzed to assess their activity against P. vorax larvae. Toxicity was low, or absent, against P. vorax for isolates, wild type cry 3Aa and cry 3Aa mutants. The genetic characterization of the collection provides opportunities for the selection of strains to be tested in bioassays against other insect pests of agricultural importance, and for designing Cry proteins with improved insecticidal toxicity.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidad de Costa Ricaen-US
dc.rightsCopyright (c) 2014 International Journal of Tropical Biology and Conservationen-US
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 57 (4) December 2009en-US
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 57 (4) December 2009es-ES
dc.sourceRevista Biología Tropical; Vol. 57 (4) December 2009pt-PT
dc.source2215-2075
dc.source0034-7744
dc.source10.15517/rbt.v57i4
dc.titlePresence and significance of Bacillus thuringiensis Cry proteins associated with the Andean weevil Premnotrypes vorax (Coleoptera: Curculionidae)en-US
dc.titlePresence and significance of Bacillus thuringiensis Cry proteins associated with the Andean weevil Premnotrypes vorax (Coleoptera: Curculionidae)es-ES
dc.typeartículo original
dc.coverageCRCen-US


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