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Vitellogenin levels in hemolymph, ovary and hepatopancreas of the freshwater crayfish Cherax quadricarinatus (Decapoda: Parastacidae) during the reproductive cycle.

dc.creatorFerré, Lilian E.
dc.creatorMedesani, Daniel A.
dc.creatorGarcía, C. Fernando
dc.creatorGrodzielski, Matías
dc.creatorRodríguez, Enrique M.
dc.date2012-10-25
dc.date.accessioned2016-05-03T15:27:12Z
dc.date.available2016-05-03T15:27:12Z
dc.identifierhttp://revistas.ucr.ac.cr/index.php/rbt/article/view/2759
dc.identifier10.15517/rbt.v60i1.2759
dc.identifier.urihttp://hdl.handle.net/10669/26113
dc.descriptionThe freshwater crayfish Cherax quadricarinatus is a tropical species of great interest for aquaculture. Vitellogenin (Vg), a lipoprotein precursor of the vitellum accumulated in spawned eggs, can be synthesized in the ovary and/or hepatopancreas of most crustaceans, being the hemolymph the way for transporting Vg throughout the reproductive cycle. Concentration of Vg in hemolymph, ovary and hepatopancreas of Cherax quadricarinatus adult females was measured by means of ELISA, specifically developed after purifying the native Vg. Measurements were made at four periods of the reproductive cycle: pre-reproductive, mid-reproductive, late reproductive and post-reproductive. Besides, both hepatosomatic (HSI) and gonadosomatic (GSI) indexes were determined in each period. Significant variations in Vg levels were detected in both hemolymph and hepatopancreas, being the highest values observed during the mid-reproductive period. Besides, such variations were positively correlated to the HSI. A positive correlation between Vg levels in hepatopancreas and ovary was also seen. These results support previous evidences about the central role of the hepatopancreas as a site of Vg synthesis in the studied species, together with the relevancy of hemolymph for transporting Vg from the hepatopancreas to the ovary. For aquaculture purposes, Vg monitoring in hemolymph could be used as a non-injurious method, to check the reproductive activity of C. quadricarinatus females.en-US
dc.descriptionLa langosta de agua dulce Cherax quadricarinatus es una especie tropical de gran interés para la acuicultura. Se midió la concentración de vitelogenina (Vg) en hemolinfa, ovario y hepatopáncreas de hembras adultas de esta especie, por medio de ELISA. Las mediciones fueron hechas en los cuatro períodos del ciclo reproductivo: pre-reproductivo, reproductivo medio, reproductivo tardío y post-reproductivo. Se detectaron variaciones significativas en los niveles de Vg tanto en hemolinfa como en hepatopáncreas, se observó el mayor valor durante el período reproductivo medio. Además, tales variaciones se correlacionaron positivamente con el índice hepatosomático. Se observó además una correlación positiva de los niveles de Vg entre hepatopáncreas y ovario. Estos resultados apoyan evidencias previas sobre el papel central del hepatopáncreas como sitio de síntesis de Vg, en esta especie, y también enfatizan la importancia de la hemolinfa para el transporte de la Vg del hepatopáncreas al ovario. Para propósitos de acuicultura, la medición de Vg en hemolinfa podría ser utilizada como un método no lesivo, con el fin de constatar la actividad reproductiva de hembras de C. quadricarinatus.es-ES
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidad de Costa Ricaen-US
dc.rightsCopyright (c) 2014 International Journal of Tropical Biology and Conservationen-US
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 60 (1) March 2012en-US
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol. 60 (1) March 2012es-ES
dc.sourceRevista Biología Tropical; Vol. 60 (1) March 2012pt-PT
dc.source2215-2075
dc.source0034-7744
dc.source10.15517/rbt.v60i1
dc.subjectcrustaceansen-US
dc.subjectreproductionen-US
dc.subjectenergy allocationen-US
dc.subjectvitellogeninen-US
dc.subjectreproductive cycleen-US
dc.subjectcrustáceoses-ES
dc.subjectreproducciónes-ES
dc.subjectinversión energéticaes-ES
dc.subjectvitelogeninaes-ES
dc.subjectciclo reproductivoes-ES
dc.titleVitellogenin levels in hemolymph, ovary and hepatopancreas of the freshwater crayfish Cherax quadricarinatus (Decapoda: Parastacidae) during the reproductive cycle.en-US
dc.titleVitellogenin levels in hemolymph, ovary and hepatopancreas of the freshwater crayfish Cherax quadricarinatus (Decapoda: Parastacidae) during the reproductive cycle.es-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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