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History, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environments

dc.creatorDíaz Ferguson, Edgardo E.
dc.creatorMoyer, Gregory R.
dc.date2014-12-01
dc.date.accessioned2016-05-03T15:27:12Z
dc.date.available2016-05-03T15:27:12Z
dc.identifierhttp://revistas.ucr.ac.cr/index.php/rbt/article/view/13231
dc.identifier10.15517/rbt.v62i4.13231
dc.identifier.urihttp://hdl.handle.net/10669/26110
dc.descriptionGenetic material (short DNA fragments) left behind by species in nonliving components of the environment (e.g. soil, sediment, or water) is defined as environmental DNA (eDNA). This DNA has been previously described as particulate DNA and has been used to detect and describe microbial communities in marine sediments since the mid-1980’s and phytoplankton communities in the water column since the early-1990’s. More recently, eDNA has been used to monitor invasive or endangered vertebrate and invertebrate species. While there is a steady increase in the applicability of eDNA as a monitoring tool, a variety of eDNA applications are emerging in fields such as forensics, population and community ecology, and taxonomy. This review provides scientist an understanding of the methods underlying eDNA detection as well as applications, key methodological considerations, and emerging areas of interest for its use in ecology and conservation of freshwater and marine environments.en
dc.descriptionGenetic material (short DNA fragments) left behind by species in nonliving components of the environment (e.g. soil, sediment, or water) is defined as environmental DNA (eDNA). This DNA has been previously described as particulate DNA and has been used to detect and describe microbial communities in marine sediments since the mid-1980’s and phytoplankton communities in the water column since the early-1990’s. More recently, eDNA has been used to monitor invasive or endangered vertebrate and invertebrate species. While there is a steady increase in the applicability of eDNA as a monitoring tool, a variety of eDNA applications are emerging in fields such as forensics, population and community ecology, and taxonomy. This review provides scientist an understanding of the methods underlying eDNA detection as well as applications, key methodological considerations, and emerging areas of interest for its use in ecology and conservation of freshwater and marine environments.es-ES
dc.formatapplication/pdf
dc.formattext/html
dc.languageeng
dc.publisherUniversidad de Costa Ricaen
dc.relationhttp://revistas.ucr.ac.cr/index.php/rbt/article/view/13231/15458
dc.rightsCopyright (c) 2014 International Journal of Tropical Biology and Conservationen
dc.sourceRevista de Biología Tropical/International Journal of Tropical Biology and Conservation; Vol 62, No 4 (2014); 1273-1284es-ES
dc.source2215-2075
dc.source0034-7744
dc.source10.15517/rbt.v62i4
dc.subjectenvironmental DNA (eDNA)en
dc.subjectdetection probabilityen
dc.subjectoccupancy modelsen
dc.subjectpersistenceen
dc.subjectmetabarcodeen
dc.subjectminibarcodeen
dc.subjectenvironmental DNA (eDNA)es-ES
dc.subjectdetection probabilityes-ES
dc.subjectoccupancy modelses-ES
dc.subjectpersistencees-ES
dc.subjectmetabarcodees-ES
dc.subjectminibarcodees-ES
dc.titleHistory, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environmentsen
dc.titleHistory, applications, methodological issues and perspectives for the use environmental DNA (eDNA) in marine and freshwater environmentses-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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