Browsing Microbiología by Author "Teixeira, Catarina de Fátima"
Now showing items 1-20 of 27
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A catalytically-inactive snake venom Lys49 phospholipase A2 homolog induces expression of cyclooxygenase-2 and production of prostaglandins through selected signaling pathways in macrophages
Moreira, Vanessa; de Castro Souto, Pollyana Cristina Maggio; Ramirez Vinolo, Marco Aurélio; Lomonte, Bruno; Gutiérrez, José María; Curi, Rui; Teixeira, Catarina de Fátima (2013-05-15)The effects of a snakevenom Lys-49phospholipaseA2 (PLA2) homolog named MT-II,devoid of enzymatic activity, on the biosynthesis of prostaglandins and protein expression of cyclooxygenase-2(COX-2) and signaling pathways ... -
A group IIA-secreted phospholipase A2 from snake venom induces lipid body formation in macrophages: the roles of intracellular phospholipases A2 and distinct signaling pathways
Leiguez, Elbio; Pavan Zuliani, Juliana; Marques Cianciarullo, Aurora; Fernandes, Cristina Maria; Gutiérrez, José María; Teixeira, Catarina de Fátima (2011-07)We investigated the ability of the sPLA(2), known as MT-III, isolated from the viperid snake Bothrops asper, to induce LB formation in macrophages and the major cellular signaling pathways involved in this process. The ... -
A Lys49 Phospholipase A2, Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region
Giannotti, Karina Cristina; Leiguez, Elbio; Moreira, Vanessa; Galvão Nascimento, Neide; Lomonte, Bruno; Gutiérrez, José María; Lopes de Melo, Robson; Teixeira, Catarina de Fátima (2013)MT-II, a Lys49PLA2 homologue devoid of catalytic activity from B. asper venom, stimulates inflammatory events in macrophages. We investigated the ability of MT-II to induce formation of lipid droplets (LDs), key elements ... -
A phospholipase A2 from Bothrops asper snake venom activates neutrophils in culture: Expression of cyclooxygenase-2 and PGE2 biosynthesis
Moreira, Vanessa; Gutiérrez, José María; Bacci Amaral, Rafaela; Lomonte, Bruno; Purgatto, Eduardo; Teixeira, Catarina de Fátima (2011-02)In this study, the production of prostaglandin E2 (PGE2) and up-regulation in cyclooxygenase (COX) pathway induced by a phospholipase A2 (PLA2), myotoxin-III (MT-III), purified from Bothrops asper snake venom, in isolated ... -
A snake venom secreted phospholipase A2 induces foam cell formation depending on the activation of factors involved in lipid homeostasis
Leiguez, Elbio; do Nascimento Viana, Mariana; Hideki Matsubara, Márcio; Fernandes, Cristina Maria; Giannotti, Karina Cristina; Gutiérrez, José María; Lomonte, Bruno; Teixeira, Catarina de Fátima (2018-06)MT-III, a snake venom GIIA sPLA2, which shares structural and functional features with mammalian GIIA sPLA2s, activates macrophage defense functions including lipid droplet (LDs) formation, organelle involved in both lipid ... -
Activation of cellular functions in macrophages by venom secretory Asp-49 and Lys-49 phospholipases A2
Zuliani, Juliana Pavan; Gutiérrez, José María; Casais e Silva, Luciana Lyra; Coccuzzo Sampaio, Sandra; Lomonte, Bruno; Teixeira, Catarina de Fátima (2005-10)The in vitro effects of myotoxin III (MT-III), an Asp-49 catalytically-active phospholipase A(2), and myotoxin II (MT-II), a catalytically-inactive Lys-49 variant, isolated from Bothrops asper snake venom, on phagocytosis ... -
An Asp49 Phospholipase A2 from Snake Venom Induces Cyclooxygenase-2 Expression and Prostaglandin E2 Production via Activation of NF-κB, p38MAPK, and PKC in Macrophages
Moreira, Vanessa; Lomonte, Bruno; Ramirez Vinolo, Marco Aurélio; Curi, Rui; Gutiérrez, José María; Teixeira, Catarina de Fátima (2014)Phospholipases A2 (PLA2) are key enzymes for production of lipid mediators. We previously demonstrated that a snake venom sPLA2 named MT-III leads to prostaglandin (PG)E2 biosynthesis in macrophages by inducing the expression ... -
Bothrops asper and Bothrops jararaca snake venoms trigger microbicidal functions of peritoneal leukocytes in vivo
Zamunér, Stella Regina; Gutiérrez, José María; Muscará, Marcelo N.; Teixeira, Simone A.; Teixeira, Catarina de Fátima (2001-10)Venoms from snakes of the genus Bothrops cause pronounced local effects in the victims. These alterations result not only from the direct toxic action of venom components, but also from the prominent inflammatory reaction ... -
Comparison of the adjuvant activity of aluminum hydroxide and calcium phosphate on the antibody response towards Bothrops asper snake venom
Olmedo, Hidekel; Herrera Vega, María; Rojas Sánchez, Leonardo; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Leiguez, Elbio; Teixeira, Catarina de Fátima; Estrada Umaña, Ricardo; Gutiérrez, José María; León Montero, Guillermo; Montero Villalobos, Mavis Lili (2014)The adjuvanticity of aluminum hydroxide and calcium phosphate on the antibody response in mice towards the venom of the snake Bothrops asper was studied. It was found that, in vitro, most of the venom proteins are similarly ... -
Critical role of TLR2 and MyD88 for functional response of macrophages to a group IIA-Secreted phospholipase A2 from snake venom
Leiguez, Elbio; Giannotti, Karina Cristina; Moreira, Vanessa; Matsubara, Marcio Hideki; Gutiérrez, José María; Lomonte, Bruno; Rodríguez, Juan Pablo; Balsinde, Jesus; Teixeira, Catarina de Fátima (PLoS One 9(4):e93741, 2014-04-09)The snake venom MT-III is a group IIA secreted phospholipase A2 (sPLA2) enzyme with functional and structural similarities with mammalian pro-inflammatory sPLA2s of the same group. Previously, we demonstrated that MT-III ... -
Effects of Bothrops asper snake venom on the expression of cyclooxygenases and production of prostaglandins by peritoneal leukocytes in vivo, and by isolated neutrophils and macrophages in vitro
Moreira, Vanessa; Gutiérrez, José María; Amaral, Rafaela Bacci; Zamunér, Stella Regina; Teixeira, Catarina de Fátima; Amaral, Rafaela Bacci (2009-03)In this study, the ability of Bothrops asper snake venom (BaV) to increase the production of prostaglandins PGE2 and PGD2 was assessed in a mouse model in vivo and in inflammatory cells in vitro. In addition, the expressions ... -
Effects of neutrophil depletion in the local pathological alterations and muscle regeneration in mice injected with Bothrops jararaca snake venom
Teixeira, Catarina de Fátima; Chaves Mora, Fernando; Zamunér, Stella Regina; Fernandes, Cristina Maria; Zuliani, Juliana Pavan; Cruz Höfling, Maria Alice; Fernandes, Irene; Gutiérrez, José María (2005-04)In order to study the role of neutrophils in the acute local pathological alterations induced by Bothrops jararaca snake venom, and in the process of skeletal muscle regeneration that follows, an experimental model was ... -
Evaluation of antivenoms in the neutralization of hyperalgesia and edema induced by Bothrops jararaca and Bothrops asper snake venoms
Picolo, Gisele; Chacur, Marucia; Gutiérrez, José María; Teixeira, Catarina de Fátima; Cury, Yara (2002-10)Neutralization of hyperalgesia induced by Bothrops jararaca and B. asper venoms was studied in rats using bothropic antivenom produced at Instituto Butantan (AVIB, 1 ml neutralizes 5 mg B. jararaca venom) and polyvalent ... -
Hyperalgesia induced by Asp49 and Lys49 phospholipases A2 from Bothrops asper snake venom: pharmacological mediation and molecular determinants
Chacur, Marucia; Longo, I.; Picolo, Gisele; Gutiérrez, José María; Lomonte, Bruno; Guerra, J. L.; Teixeira, Catarina de Fátima; Cury, Yara (2003-05)The ability of Lys49 and Asp49 phospholipases A2 (PLA2), from Bothrops asper snake venom, to cause hyperalgesia was investigated in rats, using the paw pressure test. Intraplantar injection of both toxins (5–20 μg/paw) ... -
Increments in cytokines and matrix metalloproteinases in skeletal muscle after injection of tissue-damaging toxins from the venom of the snake Bothrops asper
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Teixeira, Catarina de Fátima; Fernandes, Cristina Maria; Díaz Oreiro, Cecilia; Gutiérrez, José María (2002)Envenomations by the snake Bothrops asper are characterized by prominent local tissue damage (i.e. myonecrosis), blistering, hemorrhage and edema. Various phospholipases A2 and metalloproteinases that induce local pathological ... -
Increments in serum cytokine and nitric oxide levels in mice injected with Bothrops asper and Bothrops jararaca snake venoms
Petricevich, Vera L.; Teixeira, Catarina de Fátima; Tambourgi, Denise V.; Gutiérrez, José María (2000-09)Changes in serum levels of several cytokines and nitric oxide were studied in BALB/c mice injected intraperitoneally with one median lethal dose (LD(50)) of the venoms of Bothrops asper and Bothrops jararaca, two of the ... -
Inflammation induced by Bothrops asper venom: release of proinflammatory cytokines and eicosanoids, and role of adhesion molecules in leukocyte infiltration
Zamunér, Stella Regina; Zuliani, Juliana Pavan; Fernandes, Cristina Maria; Gutiérrez, José María; Teixeira, Catarina de Fátima (2005-12)Bothrops asper venom (BaV) causes systemic and local effects characterized by an acute inflammatory reaction with accumulation of leukocytes and release of endogenous mediators. In this study, the effects of BaV on the ... -
Inflammatory effects of BaP1 a metalloproteinase isolated from Bothrops asper snake venom: leukocyte recruitment and release of cytokines
Fernandes, Cristina Maria; Zamunér, Stella Regina; Zuliani, Juliana Pavan; Rucavado Romero, Alexandra; Gutiérrez, José María; Teixeira, Catarina de Fátima (2006-04)The inflammatory events induced by BaP1, a 22.7 kDa metalloproteinase isolated from Bothrops asper snake venom, were studied. BaP1 i.p. injection in mice induced a marked inflammatory cell infiltrate into peritoneal cavity ... -
Inflammatory events induced by Lys-49 and Asp-49 phospholipases A2 isolated from Bothrops asper snake venom: role of catalytic activity
Zuliani, Juliana Pavan; Fernandes, Cristina Maria; Zamunér, Stella Regina; Gutiérrez, José María; Teixeira, Catarina de Fátima (2005-03)The inflammatory events induced in the peritoneal cavity of mice by two PLA2s isolated from Bothrops asper snake venom were investigated. MT-III, an Asp-49 catalytically active enzyme and MT-II, a catalytically inactive ... -
Lemnitoxin, the major component of Micrurus lemniscatus coral snake venom, is a myotoxic and pro-inflammatory phospholipase A2
Casais e Silva, Luciana Lyra; Teixeira, Catarina de Fátima; Lebrun, Ivo; Lomonte, Bruno; Alape Girón, Alberto; Gutiérrez, José María (2016-08-26)The venom of Micrurus lemniscatus, a coral snake of wide geographical distribution in South America, was fractionated by reverse-phase HPLC and the fractions screened for phospholipase A2 (PLA2) activity. The major component ...