Listar Microbiología por autor "f62bf3bf-c612-451f-90ef-a366ebb3f4b5"
Mostrando ítems 21-40 de 69
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Experimental pathology of local tissue damage induced by Bothrops asper snake venom
Gutiérrez, José María; Rucavado Romero, Alexandra; Chaves Mora, Fernando; Díaz Oreiro, Cecilia; Escalante Muñoz, Teresa (2009-12-01)Envenomations by Bothrops asper are often associated with complex and severe local pathological manifestations, including edema, blistering, dermonecrosis, myonecrosis and hemorrhage. The pathogenesis of these alterations ... -
Experimental pathophysiology of systemic alterations induced by Bothrops asper snake venom
Gutiérrez, José María; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra (2009-12-01)Moderate and severe envenomations by the snake Bothrops asper provoke systemic alterations, such as systemic bleeding, coagulopathy, hypovolemia, hemodynamic instability and shock, and acute renal failure. Systemic hemorrhage ... -
Extracts of Renealmia alpinia (Rottb.) MAAS Protect against Lethality and Systemic Hemorrhage Induced by Bothrops asper Venom: Insights from a Model with Extract Administration before Venom Injection
Patiño Llano, Arley Camilo; Quintana, Juan Carlos; Gutiérrez, José María; Rucavado Romero, Alexandra; Benjumea, Dora María; Pereañez, Jaime Andrés (2015)Renealmia alpinia (Rottb.) MAAS, obtained by micropropagation (in vitro) and wild forms have previously been shown to inhibit some toxic activities of Bothrops asper snake venom if preincubated before injection. In this ... -
Hemorrhage Caused by Snake Venom Metalloproteinases: A Journey of Discovery and Understanding
Gutiérrez, José María; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Herrera Arias, Cristina (2016-03-26)The historical development of discoveries and conceptual frames for understanding the hemorrhagic activity induced by viperid snake venoms and by hemorrhagic metalloproteinases (SVMPs) present in these venoms is reviewed. ... -
Hemorrhage induced by snake venom metalloproteinases: biochemical and biophysical mechanisms involved in microvessel damage
Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Díaz Oreiro, Cecilia (2005-06-15)Zinc-dependent metalloproteinases are responsible for the hemorrhagic activity characteristic of viperid snake venoms. Snake venom metalloproteinases (SVMPs) are classified in various groups (P-I–IV), according to their ... -
Homogenates of skeletal muscle injected with snake venom inhibit myogenic differentiation in cell culture
Saravia Otten, Patricia; Robledo, Bárbara; Escalante Muñoz, Teresa; Bonilla, Llira; Rucavado Romero, Alexandra; Lomonte, Bruno; Hernández, Rosario; Flock, Jan-Ingmar; Gutiérrez, José María; Gastaldello, Stefano (2013-02)Introduction: Viperid snakebite envenomings are characterized by muscle necrosis and a deficient regenerative response. Methods: Homogenates from gastrocnemius muscles of mice injected with the venom of the snake Bothrops ... -
Identification of New Snake Venom Metalloproteinase Inhibitors Using Compound Screening and Rational Peptide Design
Villalta Romero, Fabián; Gortat, Anna; Herrera, Andrés E.; Arguedas, Rebeca; Quesada Espinoza, Francisco Javier; Lopes de Melo, Robson; Calvete Chornet, Juan José; Montero Villalobos, Mavis Lili; Murillo Masís, Renato; Rucavado Romero, Alexandra; Pérez Payá, Enrique (2012)The majority of snakebite envenomations in Central America are caused by the viperid species Bothrops asper, whose venom contains a high proportion of zinc-dependent metalloproteinases that play a relevant role in the ... -
Identification of residues critical for toxicity in Clostridium perfringens phospholipase C, the key toxin in gas gangrene
Alape Girón, Alberto; Flores Díaz, Marietta; Guillouard, Isabelle; Naylor, Claire E.; Titball, Richard; Rucavado Romero, Alexandra; Lomonte, Bruno; Basak, Ajit K.; Gutiérrez, José María; Cole, Steward T.; Thelestam, Mónica (2000-08)Clostridium perfringens phospholipase C (PLC), also called α-toxin, is the major virulence factor in the pathogenesis of gas gangrene. The toxic activities of genetically engineered α-toxin variants harboring single ... -
Immunochemical and biological characterization of monoclonal antibodies against BaP1, a metalloproteinase from Bothrops asper snake venom
Fernandes, Irene; Assumpção, G. G.; Silveira, C. R. F.; Faquim Mauro, E. L.; Tanjoni, Isabelle; Carmona, A. K.; Alves, M. F. M.; Takehara, Harumi Ando; Rucavado Romero, Alexandra; Ramos, O. H. P.; Moura Da Silva, Ana M.; Gutiérrez, José María (2010-11)BaP1 is a P-I class of Snake Venom Metalloproteinase (SVMP) relevant in the local tissue damage associated with envenomations by Bothrops asper, a medically-important species in Central America and parts of South America. ... -
Immunological studies on BaH1 and BaPI, two hemorrhagic metalloproteinases from the venom of the snake Bothrops asper
Rucavado Romero, Alexandra; Borkow, Gadi; Ovadia, Michael; Gutiérrez, José María (1995-08)No immunological cross-reactivity was observed between BaH1 and BaP1, two hemorrhagic metalloproteinases isolated from B. asper venom, by gel immunodiffusion, Western blotting and neutralization studies. Cross-reactivity ... -
Increments in cytokines and matrix metalloproteinases in skeletal muscle after injection of tissue-damaging toxins from the venom of the snake Bothrops asper
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Teixeira, Catarina de Fátima; Fernandes, Cristina Maria; Díaz Oreiro, Cecilia; Gutiérrez, José María (2002)Envenomations by the snake Bothrops asper are characterized by prominent local tissue damage (i.e. myonecrosis), blistering, hemorrhage and edema. Various phospholipases A2 and metalloproteinases that induce local pathological ... -
Inflammatory effects of BaP1 a metalloproteinase isolated from Bothrops asper snake venom: leukocyte recruitment and release of cytokines
Fernandes, Cristina Maria; Zamunér, Stella Regina; Zuliani, Juliana Pavan; Rucavado Romero, Alexandra; Gutiérrez, José María; Teixeira, Catarina de Fátima (2006-04)The inflammatory events induced by BaP1, a 22.7 kDa metalloproteinase isolated from Bothrops asper snake venom, were studied. BaP1 i.p. injection in mice induced a marked inflammatory cell infiltrate into peritoneal cavity ... -
Inhibition of local hemorrhage and dermonecrosis induced by Bothrops asper snake venom: effectiveness of early in situ administration of the peptidomimetic metalloproteinase inhibitor batimastat and the chelating agent CaNa2EDTA
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Franceschi, Aida; Chaves Mora, Fernando; León Montero, Guillermo; Cury, Yara; Ovadia, Michael; Gutiérrez, José María (2000-11)The effectiveness of the chelating agent CaNa2EDTA and the peptidomimetic matrix metalloproteinase inhibitor batimastat (BB-94) to inhibit local tissue damage induced by Bothrops asper snake venom was studied in mice. Both ... -
Isolation and biochemical, functional and structural characterization of a novel L-amino acid oxidase from Lachesis muta snake venom
Bregge Silva, Cristiane; Nonato, Maria Cristina; de Albuquerque, Sérgio; Ho, Paulo Lee; Junqueira de Azevedo, Inácio de Loiola Meirelles; Vasconcelos Diniz, Marcelo Ribeiro; Lomonte, Bruno; Rucavado Romero, Alexandra; Díaz Oreiro, Cecilia; Gutiérrez, José María; Arantes, Eliane Candiani (2012-12-01)The aim of this study was the isolation of the LAAO from Lachesis muta venom (LmLAAO) and its biochemical, functional and structural characterization. Two different purification protocols were developed and both provided ... -
Isolation and characterization of a myotoxic phospholipase A2 from the venom of the arboreal snake Bothriechis (Bothrops) schlegelii from Costa Rica
Angulo Ugalde, Yamileth; Chaves Olarte, Esteban; Rucavado Romero, Alexandra; Gutiérrez, José María; Lomonte, Bruno; Alape Girón, Alberto (1997-03)A new myotoxic phospholipase A2was isolated from the venom of the arboreal snakeBothriechis schlegelii(formerlyBothrops schlegelii) from Costa Rica, by ion-exchange chromatography on CM-Sephadex.B. schlegeliimyotoxin I is ... -
Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper
Pérez, Aida Verónica; Rucavado Romero, Alexandra; Sanz, Libia; Calvete Chornet, Juan José; Gutiérrez, José María (2008-01)A serine proteinase with thrombin-like activity was isolated from the venom of the Central American pit viper Bothrops asper. Isolation was performed by a combination of affinity chromatography on aminobenzamidine-Sepharose ... -
Isolation and characterization of four medium-size disintegrins from the venoms of Central American viperid snakes of the genera Atropoides, Bothrops, Cerrophidion and Crotalus
Angulo Ugalde, Yamileth; Castro Godínez, Adriana; Lomonte, Bruno; Rucavado Romero, Alexandra; Fernández Ulate, Julián; Calvete Chornet, Juan José; Gutiérrez, José María (2014-12)Four disintegrins were isolated from the venoms of the Central American viperid snakes Atropoides mexicanus (atropoimin), Bothrops asper (bothrasperin), Cerrophidion sasai (sasaimin), and Crotalus simus (simusmin). ... -
Key events in microvascular damage induced by snake venom hemorrhagic metalloproteinases
Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Fox, Jay W.; Gutiérrez, José María (2011-08-24)Hemorrhage is one of the most significant effects in envenomings induced by viperid snakebites. Damage to the microvasculature, induced by snake venom metalloproteinases (SVMPs), is the main event responsible for this ... -
Local and systemic pathophysiological alterations induced by a serine proteinase from the venom of the snake Bothrops jararacussu
Pérez, Aida Verónica; Saravia Otten, Patricia; Rucavado Romero, Alexandra; Sant’Ana, Carolina D.; Soares, Andreimar Martins; Gutiérrez, José María (2007-06-01)The local and systemic pathophysiological alterations induced by BjussuSP-I, a thrombin-like serine proteinase from the venom of the snake Bothrops jararacussu, were assessed in mice. BjussuSP-I induced a mild edema but ... -
Local tissue damage induced by BaP1, a metalloproteinase isolated from Bothrops asper (Terciopelo) snake venom
Rucavado Romero, Alexandra; Lomonte, Bruno; Ovadia, Michael; Gutiérrez, José María (1995-12)The pathogenesis of hemorrhage and other local effects induced by the metalloproteinase BaP1, isolated from Bothrops asper venom, was investigated using various in vivo and in vitro models. Upon intramuscular injection in ...