Listar Microbiología por autor "f85e29f0-9dae-4bc2-a3b5-eba276975db6"

Mostrando ítems 1-20 de 33

    • A new method for the detection of phospholipase A2 variants: identification of isozymes in the venoms of newborn and adult Bothrops asper (terciopelo) snakes 

      Moreno Robles, Edgardo; Alape Girón, Alberto; Sánchez, Manuel; Gutiérrez, José María (1988)
      A new method for the identification of phospholipase A2 isozymes in snake venoms is described. The technique is based on the separation of the venom components by isoelectric focusing in agarose gels, transfer of the protein ...

    • An electrophoretic study on phospholipase A2 isoenzymes in the venoms of Central American crotaline snakes 

      Valiente Álvarez, Carmen Isabel; Moreno Robles, Edgardo; Sittenfeld Appel, Ana; Lomonte, Bruno; Gutiérrez, José María (1992)
      The number and isoelectric points of phospholipase A2 isoenzymes were studied in the venoms of 12 Central American crotaline snakes of the genera Bothrops, Crotalus, Lachesis and Agkistrodon. The study was carried out by ...

    • Antibody neutralization of a myotoxin from the venom of Bothrops asper (terciopelo) 

      Lomonte, Bruno; Gutiérrez, José María; Moreno Robles, Edgardo; Cerdas Fallas, Luis (1987)
      Neutralization of biological activities of B. asper myotoxin by a monospecific anti-myotoxin rabbit serum and polyvalent antivenom was studied. Both antisera neutralized myotoxic, phospholipase A and lethal activities of ...

    • Antimicrobial activity of myotoxic phospholipases A2 from crotalid snake venoms and synthetic peptide variants derived from their C-terminal region 

      Santamaría Quesada, Carlos Manuel; Larios Ramos, Silda; Angulo Ugalde, Yamileth; Pizarro Cerdá, Javier; Gorvel, Jean Pierre; Moreno Robles, Edgardo; Lomonte, Bruno (2005)
      A short peptide derived from the C-terminal region of Bothrops asper myotoxin II, a Lys49 phospholipase A2 (PLA2), was previously found to reproduce the bactericidal activity of its parent molecule. In this study, a panel ...

    • Bactericidal activity of Lys49 and Asp49 myotoxic phospholipases A2 from Bothrops asper snake venom: synthetic Lys49 myotoxin II-(115-129)-peptide identifies its bactericidal region 

      Páramo Aguilera, Leandro Alberto; Lomonte, Bruno; Pizarro Cerdá, Javier; Bengoechea, José Antonio; Gorvel, Jean Pierre; Moreno Robles, Edgardo (1998)
      Mammalian group-II phospholipases A2 (PLA2) of inflammatory fluids display bactericidal properties, which are dependent on their enzymatic activity. This study shows that myotoxins II (Lys49) and III (Asp49), two group-II ...

    • Bactericidal and anti-endotoxic properties of short cationic peptides derived from a snake venom Lys49 phospholipase A2 

      Santamaría Quesada, Carlos Manuel; Larios Ramos, Silda; Quirós Barrantes, Steve; Pizarro Cerdá, Javier; Gorvel, Jean Pierre; Lomonte, Bruno; Moreno Robles, Edgardo (2005)
      The activities of short synthetic, nonhemolytic peptides derived from the C-terminal region of myotoxin II, a catalytically inactive phospholipase A2 homologue present in the venom of the snake Bothrops asper, have been ...

    • Brucella abortus Induces the Premature Death of Human Neutrophils through the Action of Its Lipopolysaccharide 

      Barquero Calvo, Elías; Mora Cartín, Ricardo; Arce Gorvel, Vilma; de Diego, Juana L.; Chacón Díaz, Carlos; Chaves Olarte, Esteban; Guzmán Verri, Caterina; Buret, Andre G.; Gorvel, Jean Pierre; Moreno Robles, Edgardo (2015-05-06)
      Most bacterial infections induce the activation of polymorphonuclear neutrophils (PMNs), enhance their microbicidal function, and promote the survival of these leukocytes for protracted periods of time. Brucella abortus ...

    • Brucella abortus Strain 2308 Wisconsin Genome: Importance of the Definition of Reference Strains 

      Suárez Esquivel, Marcela; Ruiz Villalobos, Nazareth; Castillo Zeledón, Amanda; Jiménez Rojas, César; Martin Roop II, Roy; Comerci, Diego J.; Barquero Calvo, Elías; Chacón Díaz, Carlos; Caswell, Clayton C.; Baker, Kate S.; Chaves Olarte, Esteban; Thomson, Nicholas R.; Moreno Robles, Edgardo; Letesson, Jean J.; De Bolle, Xavier; Guzmán Verri, Caterina (2016-09-29)
      Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research ...

    • Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts 

      Chacón Díaz, Carlos; Altamirano Silva, Pamela; González Espinoza, Gabriela; Medina, María Concepción; Alfaro Alarcón, Alejandro; Bouza Mora, Laura; Jiménez Rojas, César; Wong Araya, Melissa; Barquero Calvo, Elías; Rojas Campos, Norman; Guzmán Verri, Caterina; Moreno Robles, Edgardo; Chaves Olarte, Esteban (2015-11-10)
      Canine brucellosis caused by Brucella canis is a disease of dogs and a zoonotic risk. B. canis harbors most of the virulence determinants defined for the genus, but its pathogenic strategy remains unclear since it has not ...

    • Brucella Genetic Variability in Wildlife Marine Mammals Populations Relates to Host Preference and Ocean Distribution 

      Suárez Esquivel, Marcela; Baker, Kate S.; Ruiz Villalobos, Nazareth; Hernández Mora, Gabriela; Barquero Calvo, Elías; Castillo Zeledón, Amanda; Jiménez Rojas, César; Chacón Díaz, Carlos; Cloeckaert, Axel; Chaves Olarte, Esteban; Thomson, Nicholas R.; Moreno Robles, Edgardo; Guzmán Verri, Caterina; González Barrientos, Rocío (2017)
      Intracellular bacterial pathogens probably arose when their ancestor adapted from a free-living environment to an intracellular one, leading to clonal bacteria with smaller genomes and less sources of genetic plasticity. ...

    • Brucella neotomae Infection in Humans, Costa Rica 

      Suárez Esquivel, Marcela; Ruiz Villalobos, Nazareth; Jiménez Rojas, César; Barquero Calvo, Elías; Chacón Díaz, Carlos; Víquez Ruiz, Eunice; Rojas Campos, Norman; Baker, Kate S.; Oviedo Sánchez, Gerardo; Amuy, Ernesto; Chaves Olarte, Esteban; Thomson, Nicholas R.; Moreno Robles, Edgardo; Guzmán Verri, Caterina (2017-06)
      Several species of Brucella are known to be zoonotic, but B. neotomae infection has been thought to be limited to wood rats. In 2008 and 2011, however, B. neotomae was isolated from cerebrospinal fluid of 2 men with ...

    • Brucellosis caused by the wood rat pathogen Brucella neotomae: two case reports 

      Villalobos Vindas, Juan M.; Amuy, Ernesto; Barquero Calvo, Elías; Rojas Campos, Norman; Chacón Díaz, Carlos; Chaves Olarte, Esteban; Guzmán Verri, Caterina; Moreno Robles, Edgardo (2017-12-19)
      Background: Brucellosis is a chronic bacterial disease caused by members of the genus Brucella. Among the classical species stands Brucella neotomae, until now, a pathogen limited to wood rats. However, we have identified two ...

    • Brucellosis in mammals of Costa Rica: An epidemiological survey 

      Hernández Mora, Gabriela; Bonilla Montoya, Roberto; Barrantes Granados, Osvaldo; Esquivel Suárez, Andrea; Montero Caballero, Danilo; González Barrientos, Rocío; Fallas Monge, Zeanne; Palacios Alfaro, José David; Baldi Salas, Mario; Campos Chacón, Elena; Chanto Chacón, Grettel; Barquero Calvo, Elías; Chacón Díaz, Carlos; Chaves Olarte, Esteban; Guzmán Verri, Caterina; Romero Zúñiga, Juan José; Moreno Robles, Edgardo (2017-08-09)
      Brucellosis has been an endemic disease of cattle and humans in Costa Rica since the beginning of XX century. However, brucellosis in sheep, goats, pigs, water buffaloes, horses and cetaceans, has not been reported in the ...

    • Computational Biology in Costa Rica: the role of a small country in the global context of bioinformatics 

      Moreno Robles, Edgardo; Lomonte, Bruno; Gutiérrez, José María (2008)
      Introduction: The successful development of high throughput methods for DNA sequencing, transcriptomics, proteomics, and other –omics, has contributed to the emergence of novel possibilities for the examination of complex ...

    • Depletion of complement enhances the clearance of Brucella abortus in mice 

      González Espinoza, Gabriela; Barquero Calvo, Elías; Lizano González, Esteban; Alfaro Alarcón, Alejandro; Arias Gómez, Berny; Chaves Olarte, Esteban; Lomonte, Bruno; Moreno Robles, Edgardo; Chacón Díaz, Carlos (2018)
      Brucellosis is a bacterial disease of animals and humans. Brucella abortus barely activates the innate immune system at the onset of infection, and this bacterium is resistant to the microbicidal action of complement. ...

    • Desarrollo de las universidades públicas de Latinoamérica: una visión alternativa al modelo economicista de rentabilidad 

      Gutiérrez, José María; Moreno Robles, Edgardo (Universidad de Costa Rica, 2011)
      Los modelos ideológico-económicos preponderantes en Latinoamérica en las últimas décadas han impuesto un concepto estrecho de rentabilidad económica en muchas áreas de la sociedad, incluyendo aquellas en las que se ...

    • Detection of proteins antigenically-related to Bothrops asper myotoxin in crotaline snake venoms 

      Lomonte, Bruno; Moreno Robles, Edgardo; Gutiérrez, José María (1987)
      The presence of components antigenically related to Bothrops asper myotoxin was investigated by Western blotting and immunoelectrophoretic techniques. B. asper myotoxin is a non-glycosylated monomeric phospholipase A with ...

    • Effect of adjuvants on the antibody response of mice to Bothrops asper (Terciopelo) snake venom 

      Rucavado Romero, Alexandra; Moreno Robles, Edgardo; Gutiérrez, José María (1996)
      The adjuvant properties of several immunostimulant molecules on the murine antibody response to Bothrops asper snake venom were evaluated. Mice receiving venom together with either sodium alginate, calcium alginate, aluminum ...

    • Emergence of an Outbreak-Associated Clostridium difficile Variant with Increased Virulence 

      Quesada Gómez, Carlos; López Ureña, Diana; Chaves Olarte, Esteban; Acuña Amador, Luis Alberto; Villalobos Zúñiga, Manuel Antonio; Du, Tim; Freire, Rosemayre; Guzmán Verri, Caterina; Gamboa Coronado, María del Mar; Lawley, Trevor D.; Moreno Robles, Edgardo; Mulvey, Michael R.; de Castro Brito, Gerly Anne; Rodríguez Cavallini, Evelyn; Rodríguez Sánchez, César (2015-04)
      The prevalence of Clostridium difficile infections has increased due to the emergence of epidemic variants from diverse genetic lineages. Here we describe the emergence of a novel variant during an outbreak in a Costa Rican ...

    • Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease 

      Hernández Mora, Gabriela; Ruiz Villalobos, Nazareth; Bonilla Montoya, Roberto; Romero Zúñiga, Juan José; Jiménez Arias, Julio; González Barrientos, Rocío; Barquero Calvo, Elías; Chacón Díaz, Carlos; Rojas Campos, Norman; Chaves Olarte, Esteban; Guzmán Verri, Caterina; Moreno Robles, Edgardo (2017-08)
      Brucellosis, caused by Brucella abortus is a major disease of cattle and a zoonosis. In order to estimate the bovine brucellosis prevalence in Costa Rica (CR), a total 765 herds (13078 bovines) from six regions of CR were ...