Listar Microbiología por autor "f62bf3bf-c612-451f-90ef-a366ebb3f4b5"
Mostrando ítems 1-20 de 69
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A neutralizing recombinant single chain antibody, scFv, against BaP1, A P-I hemorrhagic metalloproteinase from Bothrops asper snake venom
Castro, J. M. A.; Oliveira, T. S.; Silveira, C. R. F.; Caporrino, M. C.; Rodriguez, D.; Moura Da Silva, Ana M.; Ramos, O. H. P.; Rucavado Romero, Alexandra; Gutiérrez, José María; Magalhães, G. S.; Faquim Mauro, E. L.; Fernandes, Irene (2014-09-01)BaP1 is a P-I class snake venom metalloproteinase (SVMP) relevant in the local tissue damage associated with envenomings by Bothrops asper, a medically important snake species in Central America and parts of South and North ... -
Amino acid sequence and crystal structure of BaP1, a metalloproteinase from Bothrops asper snake venom that exerts multiple tissue-damaging activities
Watanabe, Leandra; Shannon, John D.; Valente, Richard H.; Rucavado Romero, Alexandra; Alape Girón, Alberto; Kamiguti, Aura S.; Theakston, R. David G.; Fox, Jay W.; Gutiérrez, José María; Arni, Raghuvir K. (2003-10)BaP1 is a 22.7-kD P-I-type zinc-dependent metalloproteinase isolated from the venom of the snake Bothrops asper, a medically relevant species in Central America. This enzyme exerts multiple tissue-damaging activities, ... -
Analysis of TcdB Proteins within the Hypervirulent Clade 2 Reveals an Impact of RhoA Glucosylation on Clostridium difficile Proinflammatory Activities
Quesada Gómez, Carlos; López Ureña, Diana; Chumbler, Nicole; Kroh, Heather K.; Castro Peña, Carolina; Rodríguez Sánchez, César; Orozco Aguilar, Josué; González Camacho, Sara María; Rucavado Romero, Alexandra; Guzmán Verri, Caterina; Lawley, Trevor D.; Lacy, D. Borden; Chaves Olarte, Esteban (2016-03)Clostridium difficile strains within the hypervirulent clade 2 are responsible for nosocomial outbreaks worldwide. The increased pathogenic potential of these strains has been attributed to several factors but is still ... -
Anti-human erythrocyte antibodies in horse-derived antivenoms used in the treatment of snakebite envenomations
León Montero, Guillermo; Rodríguez, Miguel Angel; Rucavado Romero, Alexandra; Lomonte, Bruno; Gutiérrez, José María (2007-03)This work examined the presence of antibodies reacting with human erythrocytes in horse-derived antivenoms used in the treatment of snakebite envenomations, and assessed the efficacy of various fractionation protocols in ... -
Assessment of metalloproteinase inhibitors clodronate and doxycycline in the neutralization of hemorrhage and coagulopathy induced by Bothrops asper snake venom
Rucavado Romero, Alexandra; Henríquez, Mónica; García, Jonielle; Gutiérrez, José María (2008-12-01)Snake venom metalloproteinases (SVMPs) play a prominent role in the local and systemic manifestations of viperid snakebite envenomations. Thus, the possibility of using metalloproteinase inhibitors in the treatment of these ... -
Biochemical and biological characterization of two serine proteinases from Colombian Crotalus durissus cumanensis snake venom
Patiño Llano, Arley Camilo; Pereañez, Jaime Andrés; Gutiérrez, José María; Rucavado Romero, Alexandra (2013-03-01)Two clotting serine proteinases, named Cdc SI and Cdc SII, were isolated and characterized for the first time from Colombian Crotalus durissus cumanensis snake venom. The enzymes were purified using two chromatographic ... -
Blister formation and skin damage induced by BaP1, a haemorrhagic metalloproteinase from the venom of the snake Bothrops asper
Rucavado Romero, Alexandra; Núñez, Javier; Gutiérrez, José María (1998-08)Blister formation and skin damage can be induced by BaP1, a haemorrhagic metalloproteinase from the venom of the snake Bothrops asper. Pathological changes in the skin were investigated after intramuscular injections of ... -
Blood flow is required for rapid endothelial cell damage induced by a snake venom hemorrhagic metalloproteinase
Gutiérrez, José María; Núñez, Javier; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra (2006-01)The effects of blood flow interruption on the ultrastructural alterations induced by a snake venom hemorrhagic metalloproteinase on skeletal muscle capillary endothelial cells were studied. Saline solution or the ... -
Bothrops asper metalloproteinase BaP1 is inhibited by alpha(2)-macroglobulin and mouse serum and does not induce systemic hemorrhage or coagulopathy
Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Kamiguti, Aura S.; Theakston, R. David G.; Gutiérrez, José María (2004-02)The ability of the P-I metalloproteinase BaP1, isolated from the venom of the snake Bothrops asper, to induce systemic bleeding, thrombocytopenia and defibrinogenation was assessed in an experimental mouse model. Intravenous ... -
Bothrops asper snake venom and its metalloproteinase BaP–1 activate the complement system. Role in leucocyte recruitment
Farsky, Sandra H. P.; Gonçalves, Luís Roberto de Camargo; Gutiérrez, José María; Correa, Adriana P.; Rucavado Romero, Alexandra; Gasque, Philippe; Tambourgi, Denise V. (2000)The venom of the snake Bothrops asper, the most important poisonous snake in Central America, evokes an inflammatory response, the mechanisms of which are not well characterized. The objectives of this study were to ... -
Characterization of 'basparin A,' a prothrombin-activating metalloproteinase, from the venom of the snake Bothrops asper that inhibits platelet aggregation and induces defibrination and thrombosis
Loría Masís, Gilbert David; Rucavado Romero, Alexandra; Kamiguti, Aura S.; Theakston, R. David G.; Fox, Jay W.; Alape Girón, Alberto; Gutiérrez, José María (2003-10)A prothrombin activator, named ‘basparin A,’ was isolated from the venom of the crotaline snake Bothrops asper, the species responsible for the majority of snakebite cases in Central America. It is an acidic (pI 5.4), 70 ... -
Characterization of aspercetin, a platelet aggregating component from the venom of the snake Bothrops asper which induces thrombocytopenia and potentiates metalloproteinase-induced hemorrhage
Rucavado Romero, Alexandra; Soto Morera, Mónica; Kamiguti, Aura S.; Theakston, R. David G.; Fox, Jay W.; Escalante Muñoz, Teresa; Gutiérrez, José María (2001-04)Thrombocytopenia occurs in a number of patients bitten by Bothrops asper, a species responsible for the majority of snakebites in Central America and southern Mexico. In this work we describe the isolation of a new ... -
Characterization of events associated with apoptosis/anoikis induced by snake venom metalloproteinase BaP1 on human endothelial cells
Díaz Oreiro, Cecilia; Valverde, Lorena; Brenes García, Oscar Gerardo; Rucavado Romero, Alexandra; Gutiérrez, José María (2005-02)Human endothelial EA.hy926 cells were incubated with BaP1, a hemorrhagic metalloproteinase purified from Bothrops asper snake venom. Since the first hour of incubation with the proteinase, cells started showing DNA ... -
Characterization of the local tissue damage induced by LHF-II, a metalloproteinase with weak hemorrhagic activity isolated from Lachesis muta muta snake venom
Rucavado Romero, Alexandra; Flores Sánchez, Eladio; Franceschi, Aida; Magalhaes, Arinos; Gutiérrez, José María (1999-09)Local tissue damage induced by LHF-II, a 22-kDa hemorrhagic metalloproteinase from Lachesis muta venom was studied. Intravital microscopy experiments evidenced hemorrhagic events 2 min after LHF-II application onto cremaster ... -
Comparison of biochemical and cytotoxic activities of extracts obtained from dorsal spines and caudal fin of adult and juvenile non-native Caribbean lionfish (Pterois volitans/miles)
Ortiz Chaves, Natalia; Lomonte, Bruno; Rucavado Romero, Alexandra; Díaz Oreiro, Cecilia; Sáenz Espinoza, Aránzazu (2017-10)Pterois volitans/miles lionfish (adult and juvenile) dorsal spines and caudal fin extracts were compared in their general composition, enzymatic activities and hemolytic and cytotoxic effects on bovine aortic endothelial ... -
Comparison of venom composition and biological activities of the subspecies Crotalus lepidus lepidus, Crotalus lepidus klauberi and Crotalus lepidus morulus from Mexico
Martínez Romero, Gerardo; Rucavado Romero, Alexandra; Lazcano, David; Gutiérrez, José María; Borja, Miguel; Lomonte, Bruno; Garza García, Yolanda; Zugasti Cruz, Alejandro (2013-08-01)The rock rattlesnakes Crotalus lepidus comprise a group (lepidus, klauberi, morulus and maculosus) of poorly known mountain cold-tolerant snakes in Mexico. In particular, Crotalus lepidus morulus is a snake endemic of ... -
Effect of adjuvants on the antibody response of mice to Bothrops asper (Terciopelo) snake venom
Rucavado Romero, Alexandra; Moreno Robles, Edgardo; Gutiérrez, José María (1996)The adjuvant properties of several immunostimulant molecules on the murine antibody response to Bothrops asper snake venom were evaluated. Mice receiving venom together with either sodium alginate, calcium alginate, aluminum ... -
Effect of the metalloproteinase inhibitor batimastat in the systemic toxicity induced by Bothrops asper snake venom: understanding the role of metalloproteinases in envenomation
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Gutiérrez, José María (2014-03-15)The peptidomimetic hydroxamate metalloproteinase inhibitor batimastat (BB-94) was assessed for its ability to neutralize the systemic effects (lethality, hemorrhage and coagulopathy) induced by the venom of Bothrops asper, ... -
Effectiveness of batimastat, a synthetic inhibitor of matrix metalloproteinases, in neutralizing local tissue damage induced by BaP1, a hemorrhagic metalloproteinase from the venom of the snake Bothrops asper
Escalante Muñoz, Teresa; Franceschi, Aida; Rucavado Romero, Alexandra; Gutiérrez, José María (2000-07-15)Batimastat (BB-94), a synthetic hydroxamate peptidomimetic matrix metalloproteinase inhibitor, was tested for its ability to inhibit proteolytic and toxic effects induced by BaP1, a 24-kDa hemorrhagic metalloproteinase ... -
Efficacy of IgG and F(ab′)2 Antivenoms to Neutralize Snake Venom-induced Local Tissue Damage as Assessed by the Proteomic Analysis of Wound Exudate
Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Shannon, John D.; Ayala Castro, Carla N.; Villalta Arrieta, Mauren; Gutiérrez, José María; Fox, Jay W. (2012)Proteomic analysis of wound exudates represents a valuable tool to investigate tissue pathology and to assess the therapeutic success of various interventions. In this study, the ability of horse-derived IgG and F(ab0)2 ...