Listar Microbiología por autor "e12443d0-244d-4583-a307-81462144074a"
Mostrando ítems 1-20 de 34
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A randomized blinded clinical trial of two antivenoms, prepared by caprylic acid or ammonium sulphate fractionation of IgG, in Bothrops and Porthidium snake bites in Colombia: correlation between safety and biochemical characteristics of antivenoms
Otero Patiño, Rafael; Gutiérrez, José María; Rojas Céspedes, Gustavo; Núñez Rangel, Vitelbina; Díaz, Abel; Miranda, E.; Uribe, A. F.; Silva, Juan F.; Ospina, J. G.; Medina, Y.; Toro, María Fabiola; García, Martha E.; León Montero, Guillermo; García, M.; Lizano González, Sergio; De La Torre, J.; Márquez, J.; Mena, Y.; González, N.; Arenas, L. C.; Puzón, A.; Blanco, N.; Sierra, A.; Espinal, M. E.; Arboleda, Margarita; Jiménez, J. C.; Ramírez, P.; Díaz, M.; Guzmán, M. C.; Barros, J.; Henao, S.; Ramírez, A.; Macea, U.; Lozano, R. (1999-06)A randomized blinded clinical trial was performed in 53 patients bitten by Bothrops sp. and Porthidium sp. in Antioquia and Chocó, Colombia, in order to compare the efficacy and safety of two antivenoms made of whole IgG ... -
Ability of a polyvalent antivenom to neutralize the venom of Lachesis muta melanocephala, a new Costa Rican subspecies of the bushmaster
Gutiérrez, José María; Rojas Céspedes, Gustavo; Cerdas Fallas, Luis (1987)Several toxic and enzymatic activities of the venom of L. m. melanocephala were studied. This venom has many similarities with that of L. m. stenophrys, although there are quantitative differences in venom activities, as ... -
Biochemical and pharmacological similarities between the venoms of newborn Crotalus durissus durissus and adult Crotalus durissus terrificus rattlesnakes
Gutiérrez, José María; Dos Santos, María Cristina; Furtado, María de Fátima; Rojas Céspedes, Gustavo (1991)A comparative study was performed with the venoms of newborn Crotalus durissus durissus, adult Crotalus durissus terrificus and adult Crotalus durissus durissus snakes. Venom of newborn specimens of C.d. durissus is very ... -
Caprylic acid fractionation of hyperimmune horse plasma: description of a simple procedure for antivenom production
Rojas Céspedes, Gustavo; Jiménez, José Manuel; Gutiérrez, José María (1994)A simple methodology for hyperimmune horse plasma fractionation, based on caprylic acid precipitation, is described. Optimal conditions for fractionation were studied; the method gives best results when concentrated caprylic ... -
Comparative study on coagulant, defibrinating, fibrinolytic and fibrinogenolytic activities of Costa Rican crotaline snake venoms and their neutralization by a polyvalent antivenom
Gené, José Antonio; Roy Sund, Astrid; Rojas Céspedes, Gustavo; Gutiérrez, José María; Cerdas Fallas, Luis (1989)The coagulant, defibrinating, fibrino lytic and fibrinogenolytic activities of venoms from ten species of Costa Rican crotaline snakes were studied, together with the neutralization of these effects by a polyvalent antivenom. ... -
Comparative study on the ability of IgG and F(ab')2 antivenoms to neutralize lethal and myotoxic effects induced by Micrurus nigrocinctus (coral snake) venom
León Montero, Guillermo; Stiles, Bradley G.; Alape Girón, Alberto; Rojas Céspedes, Gustavo; Gutiérrez, José María (1999-08)A comparative study was performed on the ability of IgG and F(ab')2 antivenoms to neutralize lethal and myotoxic activities of Micrurus nigrocinctus venom. Both antivenoms were adjusted to a similar neutralizing potency ... -
Comparative study on the ability of IgG and Fab sheep antivenoms to neutralize local hemorrhage, edema and myonecrosis induced by Bothrops asper (terciopelo) snake venom
León Montero, Guillermo; Valverde Barrantes, Juan Manuel; Rojas Céspedes, Gustavo; Lomonte, Bruno; Gutiérrez, José María (2000-02)The ability of sheep antivenoms, consisting of whole IgG molecules or Fab fragments, to neutralize local hemorrhage, edema and myonecrosis induced by Bothrops asper venom was comparatively studied in mice. The two antivenoms ... -
Effect of Preservatives on IgG Aggregation, Complement-activating Effect and Hypotensive Activity of Horse Polyvalent Antivenom Used in Snakebite Envenomation
García González, Mildred; Monge Monge, María; León Montero, Guillermo; Lizano González, Sergio; Segura, Eduardo; Solano Trejos, María Gabriela; Rojas Céspedes, Gustavo; Gutiérrez, José María (2002-06)Intravenous administration of antivenoms is associated with early adverse reactions in a number of cases, but the causes of this phenomenon are still unclear. The effect of preservatives (phenol and thimerosal) on IgG ... -
Effect of storage temperature on the stability of polyvalent antivenom produced in Costa Rica.
Rojas Céspedes, Gustavo; Espinoza Vargas, Marlene María; Lomonte, Bruno; Gutiérrez, José María (1990)The effect of storage temperature on the stability of the liquid polyvalent (crotaline) antivenom produced at the Instituto Clodomiro Picado, Costa Rica, was studied during a twelve-month period. The following parameters ... -
Effects of a myotoxic phospholipase A2 isolated from Bothrops asper venom on skeletal muscle sarcoplasmic reticulum
Gutiérrez, José María; Rojas Céspedes, Gustavo; Lomonte, Bruno; Gené, José Antonio; Cerdas Fallas, Luis (1987)The myotoxin from B. asper snake venom inhibited Ca-ATPase activity of rabbit sarcoplasmic reticulum after incubation in vitro. Inhibition was non-competitive and albumin enhanced the effect of the toxin. Furthermore, B. ... -
Efficacy and safety of two whole IgG polyvalent antivenoms, refined by caprylic acid fractionation with or without β-propiolactone, in the treatment of Bothrops asper bites in Colombia
Otero Patiño, Rafael; León Montero, Guillermo; Gutiérrez, José María; Rojas Céspedes, Gustavo; Toro, María Fabiola; Barona, Jacqueline; Rodríguez, Verónica; Díaz, Abel; Núñez Rangel, Vitelbina; Quintana, Juan Carlos; Ayala, Shirley; Mosquera, Diana Carolina; Conrado, Lesdy L.; Fernández, Diego; Arroyo, Yobana; Piniagua, Carlos A.; López, Mercedes; Ospina, Carlos E.; Alzate, Claudia; Fernández, Jorge; Meza, Jazmín J.; Silva, Juan F.; Ramírez, Patricia; Fabra, Patricia E.; Ramírez, Eugenio; Córdoba, Elkin; Arrieta, Ana Berta; Warrell, David A.; Theakston, R. David G. (2006-12)The efficacy and safety of two whole IgG polyvalent antivenoms (A and B) were compared in a randomised, blinded clinical trial in 67 patients systemically envenomed by Bothrops asper in Colombia. Both antivenoms were ... -
Envenamiento por mordedura de serpiente en Costa Rica en 1996: Epidemiología y consideraciones clínicas
Arroyo Gutiérrez, Olga; Rojas Céspedes, Gustavo; Gutiérrez, José María (1999-12)El presente trabajo se efectúa para tener una visión actualizada y completa de las principales características epidemiológicas del envenenamiento por mordedura de serpiente en Costa Rica. Asimismo, se incluyen algunas ... -
Evaluación de la capacidad de cuatro antivenenos comerciales para neutralizar el veneno de la serpiente Bothrops asper (terciopelo) de Costa Rica
Bogarín, Guisella; Segura Agüero, Eduardo; Durán Blanco, Gina; Lomonte, Bruno; Rojas Céspedes, Gustavo; Gutiérrez, José María (1995-09)We studied the ability of four commercially available antivenoms to neutralize several toxic and enzymatic activities of Bothrops asper (terciopelo) venom from Costa Rica. Experiments with preincubation of venom and antivenom ... -
Evaluation of the neutralizing ability of antivenoms for the treatment of snake bite envenoming in Central America
Gutiérrez, José María; Rojas Céspedes, Gustavo; Bogarín, Guisella; Lomonte, Bruno (1996)De nombreuses envenimations ophidiennes en Amérique centrale sont dues aux crotales (famille des Viperidae) parmi lesquels Bothrops asper est le principal responsable. Quelques accidents sont provoqués par les serpents ... -
Experimental myonecrosis induced by the venoms of South American Micrurus (coral snakes)
Gutiérrez, José María; Rojas Céspedes, Gustavo; Da Silva, Nelson Jorge; Núñez, Javier (1992)Venoms from 11 taxa of Micrurus (coral snakes) from Brazil and Colombia were tested for myotoxic activity in mice. All venoms, except that of M. surinamensis, induced myotoxicity as judged by the increase in plasma creatine ... -
Immunoglobulin G and F(ab')2 polyvalent antivenoms do not differ in their ability to neutralize hemorrhage, edema and myonecrosis induced by Bothrops asper (terciopelo) snake venom
León Montero, Guillermo; Rojas Céspedes, Gustavo; Lomonte, Bruno; Gutiérrez, José María (1997-11)The ability of whole immunoglobulin G (IgG) and F(ab')2 polyvalent (Crotalinae) antivenoms to neutralize the hemorrhagic, edema-forming and myotoxic activities of Bothrops asper venom was studied. Both antivenoms were ... -
Inhibition by CaNa2EDTA of local tissue damage induced by Bothrops asper (terciopelo) venom: Application in horse immunization for antivenom production
León Montero, Guillermo; Estrada Umaña, Ricardo; Chaves Mora, Fernando; Rojas Céspedes, Gustavo; Ovadia, Michael; Gutiérrez, José María (1998-03-26)The ability of the chelating agent CaNa2EDTA to inhibit local tissue damage induced by Bothrops asper venom was studied in mice and in horses used for polyvalent (Crotalinae) antivenom production. CaNa2EDTA was devoid of ... -
Instituto Clodomiro Picado: Ciencia y Tecnología Endógenas en la Solución de un Problema de la Salud Pública en Centroamérica
Gutiérrez, José María; Rojas Céspedes, Gustavo (1999-05)El accidente ofídico re-presenta un importante problema de salud pública en Centroamérica. En Costa Rica, por ejemplo, ocurren alrededor de 600 mordeduras por serpientes en humanos anualmente (Gutiérrez, 1995), en tanto ... -
Isolation of a galactose-binding lectin from the venom of the snake Bothrops godmani (Godmann's pit viper).
Lomonte, Bruno; Rojas Céspedes, Gustavo; Gutiérrez, José María; Ramírez, Giselle (1990)A galactose-binding lectin, isolated from the venom of B. gotlmani by affinity chromatography . is an acidic protein (pI 4.9) with a subunit mol. wt of about 14,000, occurring mostly as a disulfide-linked dimer of 28,000. ... -
Neutralización de las actividades tóxicas y enzimáticas de cuatro venenos de serpientes de Guatemala y Honduras por el antiveneno polivalente producido en Costa Rica
Rojas Céspedes, Gustavo; Gutiérrez, José María; Gené, José Antonio; Gómez Paniagua, Maribel; Cerdas Fallas, Luis (1987)We studied the ability of the polyvalent antivenom produced in Costa Rica to neutralize lethal, hemorrhagic, edema-forming, proteolytic, hemolytic, hyaluronidase and fibrinolytic activities of the venoms of Bothrops asper ...