Listar Microbiología por autor "6a9bac5f-130f-4977-8ddf-ab97fcafacc4"
Mostrando ítems 1-20 de 418
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12-HETE is a regulator of PGE2 production via COX-2 expression induced by a snake venom group IIA phospholipase A2 in isolated peritoneal macrophages
Moreira, Vanessa; Gutiérrez, José María; Lomonte, Bruno; Ramirez Vinolo, Marco Aurélio; Curi, Rui; Lambeau, Gérard; Teixeira, Catarina de Fátima (2020)The snake venom myotoxin (MT)-III is a group IIA secreted phospholipase A2 (sPLA2) with pro-inflammatory activities. Previous studies have demonstrated that MT-III has the ability to stimulate macrophages to release ... -
A Call for Incorporating Social Research in the Global Struggle against Snakebite
Gutiérrez, José María; Burnouf, Thierry; Harrison, Robert A.; Calvete Chornet, Juan José; Brown, Nicholas I.; Jensen, Simon D.; Warrell, David A.; Williams, David J. (2015-09-17)In Africa, Asia, Latin America, and parts of Oceania, envenoming after snakebite is a serious public health problem. Conservative data suggest that between 1.2 and 5.5 million people suffer snakebites every year, resulting ... -
A catalytically-inactive snake venom Lys49 phospholipase A2 homolog induces expression of cyclooxygenase-2 and production of prostaglandins through selected signaling pathways in macrophages
Moreira, Vanessa; de Castro Souto, Pollyana Cristina Maggio; Ramirez Vinolo, Marco Aurélio; Lomonte, Bruno; Gutiérrez, José María; Curi, Rui; Teixeira, Catarina de Fátima (2013-05-15)The effects of a snakevenom Lys-49phospholipaseA2 (PLA2) homolog named MT-II,devoid of enzymatic activity, on the biosynthesis of prostaglandins and protein expression of cyclooxygenase-2(COX-2) and signaling pathways ... -
A cellular deficiency of gangliosides causes hypersensitivity to Clostridium perfringens phospholipase C
Flores Díaz, Marietta; Alape Girón, Alberto; Clark, Graeme; Catimel, Bruno; Hirabayashi, Yoshio; Nice, Ed; Gutiérrez, José María; Titball, Richard; Thelestam, Mónica (2005-07-22)Clostridium perfringens phospholipase C (Cp-PLC), also called alpha-toxin, is the major virulence factor in the pathogenesis of gas gangrene. Previously, a cellular UDP-Glc deficiency was related with a hypersensitivity ... -
A comparison of in vitro methods for assessing the potency of therapeutic antisera against the venom of the coral snake Micrurus nigrocinctus
Alape Girón, Alberto; Miranda Arrieta, Keyna; Cortés Bratti, Ximena; Stiles, Bradley G.; Gutiérrez, José María (1997-04)Therapeutic antisera against Micrurus nigrocinctus venom were tested for protection against lethality, as well as for inhibition of the nicotinic acetylcholine receptor (AchR)-binding and neutralization of phospholipase ... -
A eficácia do antiveneno botrópico-crotálico na neutralização das principais atividades do veneno de Bothrops jararacussu
Dos Santos, María Cristina; Gonçalves, Luís Roberto de Camargo; Fortes Dias, Consuelo L.; Cury, Yara; Gutiérrez, José María; Furtado, María de Fátima (1992)Myonecrosis is one of the effects of Bothrops jararacussu venom, from which a myotoxin was isolated showing structural homology to phospholipase A2 (PLA2), but without enzimatic activity. Such myotoxic activity is also ... -
A group IIA-secreted phospholipase A2 from snake venom induces lipid body formation in macrophages: the roles of intracellular phospholipases A2 and distinct signaling pathways
Leiguez, Elbio; Pavan Zuliani, Juliana; Marques Cianciarullo, Aurora; Fernandes, Cristina Maria; Gutiérrez, José María; Teixeira, Catarina de Fátima (2011-07)We investigated the ability of the sPLA(2), known as MT-III, isolated from the viperid snake Bothrops asper, to induce LB formation in macrophages and the major cellular signaling pathways involved in this process. The ... -
A lipidomic perspective of the action of group IIA secreted phospholipase A2 on human monocytes: lipid droplet biogenesis and activation of cytosolic phospholipase A2α
Rodríguez, Juan Pablo; Leiguez, Elbio; Guijas, Carlos; Lomonte, Bruno; Gutiérrez, José María; Teixeira, Catarina de Fátima; Balboa, María A.; Balsinde Rodríguez, Jesús (2020)Phospholipase A2s constitute a wide group of lipid-modifying enzymes which display a variety of functions in innate immune responses. In this work, we utilized mass spectrometry-based lipidomic approaches to investigate ... -
A Lys49 phospholipase A2 homologue from Bothrops asper snake venom induces proliferation, apoptosis and necrosis in a lymphoblastoid cell line
Mora Rodríguez, Rodrigo Antonio; Valverde Rojas, Berta E.; Díaz Oreiro, Cecilia; Lomonte, Bruno; Gutiérrez, José María (2005-04)Lys49 phospholipase A2 homologues are abundant in viperid snake venoms. These proteins have substitutions at the calcium-binding loop and catalytic center which render them enzymatically inactive; however, they display a ... -
A Lys49 Phospholipase A2, Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region
Giannotti, Karina Cristina; Leiguez, Elbio; Moreira, Vanessa; Galvão Nascimento, Neide; Lomonte, Bruno; Gutiérrez, José María; Lopes de Melo, Robson; Teixeira, Catarina de Fátima (2013)MT-II, a Lys49PLA2 homologue devoid of catalytic activity from B. asper venom, stimulates inflammatory events in macrophages. We investigated the ability of MT-II to induce formation of lipid droplets (LDs), key elements ... -
A Lys49-PLA2 myotoxin of Bothrops asper triggers a rapid death of macrophages that involves autocrine purinergic receptor signaling
Tonello, Fiorella; Simonato, M.; Aita, A.; Pizzo, Paola; Fernández Ulate, Julián; Lomonte, Bruno; Gutiérrez, José María; Montecucco, Cesare (Cell Death and Disease (2012) 3, e343, 2012-07-05)Lys49-PLA2 myotoxins, an important component of various viperid snake venoms, are a class of PLA2-homolog proteins deprived of catalytic activity. Similar to enzymatically active PLA2 (Asp49) and to other classes of ... -
A multicomponent strategy to improve the availability of antivenom for treating snakebite envenoming
Gutiérrez, José María; Burnouf, Thierry; Harrison, Robert A.; Calvete Chornet, Juan José; Kuch, Ulrich; Warrell, David A.; Williams, David J. (2014-07-01)Snakebite envenoming is a common but neglected public health problem, particularly in impoverished rural regions of sub-Saharan Africa, Asia and Latin America. The only validated treatment for this condition is passive ... -
A neutralizing recombinant single chain antibody, scFv, against BaP1, A P-I hemorrhagic metalloproteinase from Bothrops asper snake venom
Castro, J. M. A.; Oliveira, T. S.; Silveira, C. R. F.; Caporrino, M. C.; Rodriguez, D.; Moura Da Silva, Ana M.; Ramos, O. H. P.; Rucavado Romero, Alexandra; Gutiérrez, José María; Magalhães, G. S.; Faquim Mauro, E. L.; Fernandes, Irene (2014-09-01)BaP1 is a P-I class snake venom metalloproteinase (SVMP) relevant in the local tissue damage associated with envenomings by Bothrops asper, a medically important snake species in Central America and parts of South and North ... -
A new method for the detection of phospholipase A2 variants: identification of isozymes in the venoms of newborn and adult Bothrops asper (terciopelo) snakes
Moreno Robles, Edgardo; Alape Girón, Alberto; Sánchez, Manuel; Gutiérrez, José María (1988)A new method for the identification of phospholipase A2 isozymes in snake venoms is described. The technique is based on the separation of the venom components by isoelectric focusing in agarose gels, transfer of the protein ... -
A new muscle damaging toxin, myotoxin II, from the venom of the snake Bothrops asper (terciopelo)
Lomonte, Bruno; Gutiérrez, José María (1989)A new muscle damaging toxin, myotoxin II, was purified from the venom of Bothrops asper by ion-exchange chromatography on CMSephadex C-25. The toxin is a dimeric, basic protein with a monomer mol.wt of 13,341, according ... -
A new Pan African polyspecific antivenom developed in response to the antivenom crisis in Africa
Laing, Gavin D.; Renjifo, J.M.; Ruiz, F.; Harrison, Robert A.; Nasidi, Abdulsalami; Gutiérrez, José María; Rowley, Paul D.; Warrell, David A.; Theakston, R. David G. (2003-07)Currently there is a crisis in the supply of antivenom for treatment of snake bite in sub-Saharan Africa. Commercial pressures have resulted in the reduction or even cessation of production of antivenom by European ... -
A novel pentameric phospholipase A2 myotoxin (PophPLA2) from the venom of the pit viper Porthidium ophryomegas
Vindas González, Julio; Carrera Rengifo, Yarina; Lomonte, Bruno; Gutiérrez, José María; Calvete Chornet, Juan José; Sanz, Libia; Fernández Ulate, Julián (2018-06-07)The first toxin isolated from the venomous pit viper Porthidiumophryomegas is a basic pentameric phospholipase A2 (PophPLA2). Elucidation of its amino acid sequence showed that it belongs to the group IIA of secreted ... -
A phospholipase A2 from Bothrops asper snake venom activates neutrophils in culture: Expression of cyclooxygenase-2 and PGE2 biosynthesis
Moreira, Vanessa; Gutiérrez, José María; Bacci Amaral, Rafaela; Lomonte, Bruno; Purgatto, Eduardo; Teixeira, Catarina de Fátima (2011-02)In this study, the production of prostaglandin E2 (PGE2) and up-regulation in cyclooxygenase (COX) pathway induced by a phospholipase A2 (PLA2), myotoxin-III (MT-III), purified from Bothrops asper snake venom, in isolated ... -
A potent antihemorrhagin in the serum of the non-poisonous water snake Natrix tessellata: isolation, characterization and mechanism of neutralization
Borkow, Gadi; Gutiérrez, José María; Ovadia, Michael (1994-12)The main natural antihemorrhagic factor (NtAH), which inhibits the hemorrhagic activity of Bothrops asper snake venom, was isolated from the serum of the non-poisonous water snake Natrix tessellata by ammonium sulfate ... -
A randomized blinded clinical trial of two antivenoms, prepared by caprylic acid or ammonium sulphate fractionation of IgG, in Bothrops and Porthidium snake bites in Colombia: correlation between safety and biochemical characteristics of antivenoms
Otero Patiño, Rafael; Gutiérrez, José María; Rojas Céspedes, Gustavo; Núñez Rangel, Vitelbina; Díaz, Abel; Miranda, E.; Uribe, A. F.; Silva, Juan F.; Ospina, J. G.; Medina, Y.; Toro, María Fabiola; García, Martha E.; León Montero, Guillermo; García, M.; Lizano González, Sergio; De La Torre, J.; Márquez, J.; Mena, Y.; González, N.; Arenas, L. C.; Puzón, A.; Blanco, N.; Sierra, A.; Espinal, M. E.; Arboleda, Margarita; Jiménez, J. C.; Ramírez, P.; Díaz, M.; Guzmán, M. C.; Barros, J.; Henao, S.; Ramírez, A.; Macea, U.; Lozano, R. (1999-06)A randomized blinded clinical trial was performed in 53 patients bitten by Bothrops sp. and Porthidium sp. in Antioquia and Chocó, Colombia, in order to compare the efficacy and safety of two antivenoms made of whole IgG ...