Listar Microbiología por autor "6657d56d-085a-41f5-ad67-1a3e2cd88702"
Mostrando ítems 1-20 de 22
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A randomized blinded clinical trial of two antivenoms, prepared by caprylic acid or ammonium sulphate fractionation of IgG, in Bothrops and Porthidium snake bites in Colombia: correlation between safety and biochemical characteristics of antivenoms
Otero Patiño, Rafael; Gutiérrez, José María; Rojas Céspedes, Gustavo; Núñez Rangel, Vitelbina; Díaz, Abel; Miranda, E.; Uribe, A. F.; Silva, Juan F.; Ospina, J. G.; Medina, Y.; Toro, María Fabiola; García, Martha E.; León Montero, Guillermo; García, M.; Lizano González, Sergio; De La Torre, J.; Márquez, J.; Mena, Y.; González, N.; Arenas, L. C.; Puzón, A.; Blanco, N.; Sierra, A.; Espinal, M. E.; Arboleda, Margarita; Jiménez, J. C.; Ramírez, P.; Díaz, M.; Guzmán, M. C.; Barros, J.; Henao, S.; Ramírez, A.; Macea, U.; Lozano, R. (1999-06)A randomized blinded clinical trial was performed in 53 patients bitten by Bothrops sp. and Porthidium sp. in Antioquia and Chocó, Colombia, in order to compare the efficacy and safety of two antivenoms made of whole IgG ... -
A randomized double-blind clinical trial of two antivenoms in patients bitten by Bothrops atrox in Colombia
Otero Patiño, Rafael; Gutiérrez, José María; Núñez Rangel, Vitelbina; Robles, Abel; Estrada Umaña, Ricardo; Segura, Eduardo; Toro, María Fabiola; García, M. E.; Díaz, Abel; Ramírez, Eugenio; Gómez, G.; Castañeda, J.; Moreno, M. E. (1996-12)A randomized double-blind clinical trial in 39 patients envenomed by Bothrops atrox in Antioquia and Chocó, Colombia, was performed to compare the efficacy and safety of 2 equine-derived antivenoms prepared at Instituto ... -
Ability of six Latin American antivenoms to neutralize the venom of mapaná equis (Bothrops atrox) from Antioquia and Chocó (Colombia)
Otero Patiño, Rafael; Núñez Rangel, Vitelbina; Osorio, Raúl Guillermo; Gutiérrez, José María; Giraldo, Cesar Augusto; Posada, Luz Elena (1995-06)This investigation compared the ability of six Latin American antivenoms (monovalent antibothropic INS, Santafé de Bogotá; polyvalent INS; polyvalent probiol, Santafé de Bogotá; antibothropic Instituto Butantan, IB, São ... -
An acidic phospholipase A2 with antibacterial activity from Porthidium nasutum snake venom
Vargas Muñoz, Leidy Johana; Londoño Monsalve, Mónica; Quintana Castillo, Juan Carlos; Rua, Carolina; Segura Latorre, Cesar; Lomonte, Bruno; Núñez Rangel, Vitelbina (2012-04)Snake venoms are complex mixtures of proteins among which both basic and acidic phospholipases A2 (PLA2s) can be found. Basic PLA2s are usually responsible for major toxic effects induced by snake venoms, while acidic ... -
Antibodies against a single fraction of Micrurus dumerilii venom neutralize the lethal effect of whole venom
Gómez Robles, Jeisson; Rey Suárez, Paola; Pereañez, Jaime Andrés; Lomonte, Bruno; Núñez Rangel, Vitelbina (2023-02-01)The coralsnake Micrurus dumerilii (Elapidae) is reported to cause envenomings of medical importance. Previous studies characterized the protein composition of its venom, with phospholipase A2 (PLA2) proteins the most ... -
Comparative study of the venoms of three subspecies of Lachesis muta (bushmaster) from Brazil, Colombia and Costa Rica
Otero Patiño, Rafael; Furtado, María de Fátima; Gonçalves, Luís Roberto de Camargo; Núñez Rangel, Vitelbina; García, Martha E.; Osorio, Raúl Guillermo; Romero, Marjorie; Gutiérrez, José María (1998-12)A comparative study was performed on the pharmacology and biochemistry of venoms from three subspecies of Lachesis muta (L. m. stenophrys, L. m. muta and L. m. rhombeata) from Brazil, Colombia and Costa Rica. All venoms ... -
Efficacy and safety of two whole IgG polyvalent antivenoms, refined by caprylic acid fractionation with or without β-propiolactone, in the treatment of Bothrops asper bites in Colombia
Otero Patiño, Rafael; León Montero, Guillermo; Gutiérrez, José María; Rojas Céspedes, Gustavo; Toro, María Fabiola; Barona, Jacqueline; Rodríguez, Verónica; Díaz, Abel; Núñez Rangel, Vitelbina; Quintana, Juan Carlos; Ayala, Shirley; Mosquera, Diana Carolina; Conrado, Lesdy L.; Fernández, Diego; Arroyo, Yobana; Piniagua, Carlos A.; López, Mercedes; Ospina, Carlos E.; Alzate, Claudia; Fernández, Jorge; Meza, Jazmín J.; Silva, Juan F.; Ramírez, Patricia; Fabra, Patricia E.; Ramírez, Eugenio; Córdoba, Elkin; Arrieta, Ana Berta; Warrell, David A.; Theakston, R. David G. (2006-12)The efficacy and safety of two whole IgG polyvalent antivenoms (A and B) were compared in a randomised, blinded clinical trial in 67 patients systemically envenomed by Bothrops asper in Colombia. Both antivenoms were ... -
Inhibitory effects of Piper umbellatum and Piper peltatum extracts towards myotoxic phospholipases A2 from Bothrops snake venoms: isolation of 4-nerolidylcatechol as active principle
Núñez Rangel, Vitelbina; Castro Araya, Víctor H.; Murillo Masís, Renato; Ponce Soto, Luis Alberto; Merfort, Irmgard; Lomonte, Bruno (2005)Phospholipases A2 (PLA2) are important constituents of snake venoms, being responsible for several of their toxic actions. Extracts from plants used in folk medicine were screened for inhibition of the enzymatic activity ... -
Integrative characterization of the venom of the coral snake Micrurus dumerilii (Elapidae) from Colombia: proteome, toxicity, and cross-neutralization by antivenom
Rey Suárez, Paola; Núñez Rangel, Vitelbina; Fernández Ulate, Julián; Lomonte, Bruno (2016-03)In Colombia, nearly 2.8% of the 4200 snakebite accidents recorded annually are inflicted by coral snakes (genus Micrurus). Micrurus dumerilii has a broad distribution in this country, especially in densely populated ... -
Mipartoxin-I, a novel three-finger toxin, is the major neurotoxic component in the venom of the redtail coral snake Micrurus mipartitus (Elapidae)
Rey Suárez, Paola; Floriano, Rafael Stuani; Rostelato Ferreira, Sandro; Saldarriaga Córdoba, Mónica María; Núñez Rangel, Vitelbina; Rodrigues Simioni, Lea; Lomonte, Bruno (2012)The major venom component of Micrurus mipartitus, a coral snake distributed from Nicaragua to northern South America, was characterized biochemically and functionally. This protein, named mipartoxin-I, is a novel member ... -
MipLAAO, a new L-amino acid oxidase from the redtail coral snake, Micrurus mipartitus
Rey Suárez, Paola; Acosta, Cristian; Torres Lamus, Uday Daniel; Saldarriaga Córdoba, Mónica María; Lomonte, Bruno; Núñez Rangel, Vitelbina (2018-06-08)L-amino acid oxidases (LAAOs) are ubiquitous enzymes in nature. Bioactivities described for these enzymes include apoptosis induction, edema formation, induction or inhibition of platelet aggregation, as well as antiviral, ... -
Neutralization of Bothrops asper venom by antibodies, natural products, and synthetic drugs: contributions to understanding snakebite envenomings and their treatment
Lomonte, Bruno; León Montero, Guillermo; Angulo Ugalde, Yamileth; Rucavado Romero, Alexandra; Núñez Rangel, Vitelbina (2009)Interest in studies on the neutralization of snake venoms and toxins by diverse types of inhibitors is two-fold. From an applied perspective, results enclose the potential to be translated into useful therapeutic products ... -
Neutralizing capacity of a new monovalent anti-Bothrops atrox antivenom: Comparison with two commercial antivenoms
Otero Patiño, Rafael; Núñez Rangel, Vitelbina; Gutiérrez, José María; Robles, Abel; Estrada Umaña, Ricardo; Osorio, Raúl Guillermo; Del Valle, G.; Valderrama, R.; Giraldo, Cesar Augusto (1997-04)Three horse-derived antivenoms were tested for their ability to neutralize lethal, hemorrhagic, edema-forming, defibrinating and myotoxic activities induced by the venom of Bothrops atr-ox from Antioquia and Choco (Colombia). ... -
Novel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus coral snake venoms: phylogenetic relationships and characterization of Clarkitoxin-I-Mdum
Rey Suárez, Paola; Saldarriaga Córdoba, Mónica María; Torres Lamus, Uday Daniel; Marin Villa, Marcel; Lomonte, Bruno; Núñez Rangel, Vitelbina (2019)Micrurus mipartitus and M. dumerilii are the most medically important coral snakes in Colombia. Proteomic characterization of their venoms has previously shown that proteins of the three-finger toxin (3FTx) family ... -
Ontogenetic variability of Bothrops atrox and Bothrops asper snake venoms from Colombia
Saldarriaga Córdoba, Mónica María; Otero Patiño, Rafael; Núñez Rangel, Vitelbina; Toro, María Fabiola; Dı́az, Abel; Gutiérrez, José María (2003-09)The lancehead snakes Bothrops asper and Bothrops atrox inflict 70–90% of the 3000 bites reported every year in Colombia. In this work, the venoms of B. atrox from Meta (Villavicencio, 33 specimens) and B. asper from Antioquia ... -
Preclinical assessment of the neutralizing capacity of antivenoms produced in six Latin American countries against medically-relevant Bothrops snake venoms
Segura Ruiz, Álvaro; Castillo, M. C.; Núñez Rangel, Vitelbina; Yarlequé, A.; Gonçalves, Luís Roberto de Camargo; Villalta Arrieta, Mauren; Bonilla, César; Herrera Vega, María; Fernández, M.; Yano, M. Y.; Araújo, H. P.; Boller, M. A. A.; León, P.; Sano Martins, I. S.; Gómez, A.; Fernández, Gil Patrick; Geoghegan, Patricia; Higashi, Hisako Gondo; León Montero, Guillermo; Gutiérrez, José María; Tintaya, Benigno (2010-11)Species of the genus Bothrops induce the vast majority of snakebite envenomings in Latin America. A preclinical study was performed in the context of a regional network of public laboratories involved in the production, ... -
Primary structures and partial toxicological characterization of two phospholipases A2 from Micrurus mipartitus and Micrurus dumerilii coral snake venoms
Rey Suárez, Paola; Núñez Rangel, Vitelbina; Saldarriaga Córdoba, Mónica María; Lomonte, Bruno (2017-06)Snake venom phospholipases A2 (PLA2) share high sequence identities and a conserved structural scaffold, but show important functional differences. Only a few PLA2s have been purified and characterized from coral snake ... -
Proteomic and biological characterization of the venom of the redtail coral snake, Micrurus mipartitus (Elapidae), from Colombia and Costa Rica
Rey Suárez, Paola; Núñez Rangel, Vitelbina; Gutiérrez, José María; Lomonte, Bruno (2011-12)Venoms of the redtail coral snake Micrurus mipartitus from Colombia and Costa Rica were analyzed by “venomics”, a proteomic strategy to determine their composition. Proteins were separated by RP-HPLC, followed by SDS-PAGE, ... -
Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism
Núñez Rangel, Vitelbina; Cid, Pedro; Sanz, Libia; De La Torre, Pilar; Angulo Ugalde, Yamileth; Lomonte, Bruno; Gutiérrez, José María; Calvete Chornet, Juan José (2009-11-02)The venom proteomes of Bothrops atrox from Colombia, Brazil, Ecuador, and Perú were characterized using venomic and antivenomic strategies. Our results evidence the existence of two geographically differentiated venom ... -
Snake venomics of Lachesis muta rhombeata and genus-wide antivenomics assessment of the paraspecific immunoreactivity of two antivenoms evidence the high compositional and immunological conservation across Lachesis
Pla Ferrer, Davinia; Sanz, Libia; Molina Sánchez, Pedro; Zorita, Virginia; Madrigal Villalobos, Marvin; Flores Díaz, Marietta; Alape Girón, Alberto; Núñez Rangel, Vitelbina; Andrés, Vicente; Gutiérrez, José María; Calvete Chornet, Juan José (2013-08-26)We report the proteomic analysis of the Atlantic bushmaster, Lachesis muta rhombeata, from Brazil. Along with previous characterization of the venom proteomes of L. stenophrys (Costa Rica), L. melanocephala (Costa Rica), ...