Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya

De, Kaylan K.; Sil, Sujit

dc.creator	De, Kaylan K.
dc.creator	Sil, Sujit
dc.date	2015-08-05
dc.date.accessioned	2016-05-02T22:15:51Z
dc.date.available	2016-05-02T22:15:51Z
dc.identifier	http://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/20747
dc.identifier	10.15517/lank.v15i2.20747
dc.identifier.uri	https://hdl.handle.net/10669/21249
dc.description	An efficient induction of protocrom-like bodies (PLBs) and plantlet regeneration from the young leaves of in vitro grown seedlings of Coelogyne flaccida, an horticulturally and medicinally important endangered epiphytic orchid, was accomplished in order to develop mass-scale propagation. The young leaves (1.5 cm in length) from 110 days old aseptically germinated seedling were grown in vitro in Murashige and Skoog’s (MS) medium supplemented with different concentrations and combinations of NAA ( 0.5-2 mg/L), BAP (0.5-2 mg/L) and Kn ( 0.5-2 mg/L ). The explants produced protocorm-like bodies directly from the epidermal cells of leaf without the formation of intervening callus tissue within four weeks of culture. The highest number of plantlets regenerated through PLBs per explant after 15 weeks was 35-36 in presence of NAA (2mg/l) and Kn (2mg/l). Within 20-25 weeks individual plantlets produced 2-3 leaves and 2-3 roots. Chromosome number from all plants regenerated from leaf explants showed the same chromosome number as the mother plant as 2n = 40. During acclimatization, 80% of the plantlets survived after one month of transplantation.	es-ES
dc.description	An efficient induction of protocrom-like bodies (PLBs) and plantlet regeneration from the young leaves of in vitro grown seedlings of Coelogyne flaccida, an horticulturally and medicinally important endangered epiphytic orchid, was accomplished in order to develop mass-scale propagation. The young leaves (1.5 cm in length) from 110 days old aseptically germinated seedling were grown in vitro in Murashige and Skoog’s (MS) medium supplemented with different concentrations and combinations of NAA ( 0.5-2 mg/L), BAP (0.5-2 mg/L) and Kn ( 0.5-2 mg/L ). The explants produced protocorm-like bodies directly from the epidermal cells of leaf without the formation of intervening callus tissue within four weeks of culture. The highest number of plantlets regenerated through PLBs per explant after 15 weeks was 35-36 in presence of NAA (2mg/l) and Kn (2mg/l). Within 20-25 weeks individual plantlets produced 2-3 leaves and 2-3 roots. Chromosome number from all plants regenerated from leaf explants showed the same chromosome number as the mother plant as 2n = 40. During acclimatization, 80% of the plantlets survived after one month of transplantation.	en-US
dc.format	application/pdf
dc.language	spa
dc.publisher	Universidad de Costa Rica	es-ES
dc.relation	Lankesteriana;
dc.rights	Copyright (c) 2015 Lankesteriana	es-ES
dc.source	Lankesteriana; Lankesteriana: Volumen 15, Número 2	es-ES
dc.source	2215-2067
dc.source	1409-3871
dc.subject	Coelogyne flaccida	es-ES
dc.subject	foliar explant	es-ES
dc.subject	micropropagation	es-ES
dc.subject	Coelogyne flaccida	en-US
dc.subject	foliar explant	en-US
dc.subject	micropropagation	en-US
dc.title	Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya	es-ES
dc.title	Protocorm-like bodies and plant regeneration from foliar explants of Coelogyne flaccida, a horticulturally and medicinally important endangered orchid of Eastern Himalaya	en-US
dc.type	artículo original

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