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Propagation of Dendrobium aggregatum by green capsule culture

dc.creatorVijayakumar, S.
dc.creatorRajalkshmi, G.
dc.creatorKalimuthu, K.
dc.date2013-09-04
dc.date.accessioned2016-05-02T22:14:51Z
dc.date.available2016-05-02T22:14:51Z
dc.identifierhttp://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763
dc.identifier10.15517/lank.v0i0.11763
dc.identifier.urihttp://hdl.handle.net/10669/20990
dc.descriptionAn efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.en-US
dc.descriptionAn efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.es-ES
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidad de Costa Ricaes-ES
dc.relationLankesteriana;
dc.rightsCopyright (c) 2014 Lankesterianaes-ES
dc.sourceLankesteriana; Lankesteriana: Volumen 12, Número 2es-ES
dc.source2215-2067
dc.source1409-3871
dc.subjectdendrobiumen-US
dc.subjectprotocorm like bodiesen-US
dc.subjectacclimatizationen-US
dc.subjectin vitroen-US
dc.subjectdendrobiumes-ES
dc.subjectprotocorm like bodieses-ES
dc.subjectacclimatizationes-ES
dc.subjectin vitroes-ES
dc.titlePropagation of Dendrobium aggregatum by green capsule cultureen-US
dc.titlePropagation of Dendrobium aggregatum by green capsule culturees-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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