Propagation of Dendrobium aggregatum by green capsule culture

Vijayakumar, S.; Rajalkshmi, G.; Kalimuthu, K.

dc.creator	Vijayakumar, S.
dc.creator	Rajalkshmi, G.
dc.creator	Kalimuthu, K.
dc.date	2013-09-04
dc.date.accessioned	2016-05-02T22:14:51Z
dc.date.available	2016-05-02T22:14:51Z
dc.identifier	http://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763
dc.identifier	10.15517/lank.v0i0.11763
dc.identifier.uri	https://hdl.handle.net/10669/20990
dc.description	An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.	en-US
dc.description	An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.	es-ES
dc.format	application/pdf
dc.language	eng
dc.publisher	Universidad de Costa Rica	es-ES
dc.relation	Lankesteriana;
dc.rights	Copyright (c) 2014 Lankesteriana	es-ES
dc.source	Lankesteriana; Lankesteriana: Volumen 12, Número 2	es-ES
dc.source	2215-2067
dc.source	1409-3871
dc.subject	dendrobium	en-US
dc.subject	protocorm like bodies	en-US
dc.subject	acclimatization	en-US
dc.subject	in vitro	en-US
dc.subject	dendrobium	es-ES
dc.subject	protocorm like bodies	es-ES
dc.subject	acclimatization	es-ES
dc.subject	in vitro	es-ES
dc.title	Propagation of Dendrobium aggregatum by green capsule culture	en-US
dc.title	Propagation of Dendrobium aggregatum by green capsule culture	es-ES
dc.type	artículo original

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