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dc.creatorAchí Araya, María Rosario
dc.creatorLindberg, Alf A.
dc.date.accessioned2015-08-05T17:05:32Z
dc.date.available2015-08-05T17:05:32Z
dc.date.issued1996-08
dc.identifier.issn1198-743X
dc.identifier.urihttp://hdl.handle.net/10669/15166
dc.descriptionReporte técnico -- Universidad de Costa Rica, Instituto de Investigaciones en Salud. 1996. Este documento es privado debido a limitaciones de derechos de autor.es_ES
dc.description.abstractOBJECTIVE: The aim of the present work was to develop a rapid, specific and highly sensitive diagnostic method for the detection of Shigella sonnei directly from stool samples. DESIGN AND METHODS: An immunomagnetic separation-polymerase chain reaction (IMS-PCR) combined assay for diagnosis of S. sonnei was developed. For this, a monoclonal antibody (Mab) specific for the O-antigen of S. sonnei was coated to magnetic beads for capture, concentration and separation of S. sonnei from feces. Bacterial DNA, was amplified by the PCR with specific primers. The amplified products were developed by dot blot hybridization with a specific alkaline phosphatase-conjugated probe. RESULTS: The purified MASS MAb reacted specifically with the Plesiomonas shigelloides (the same O-antigen as Shigella sonnei) LPS. The primers were specific for invasive Shigella and enteroinvasive Escherichia coli. Only invasive Shigella sonnei strains gave a positive result in the IMS-PCR assay. The detection limit was 10 to 15 c.f.u. CONCLUSIONS: The availability of IMS-PCR assays provides an improved method for the diagnosis of shigellae directly from feces. The assay is rapid, highly sensitive and specific for the detection of Shigella sonnei directly from stool samples.es_ES
dc.description.sponsorshipUniversidad de Costa Rica, Instituto de Investigaciones en Saludes_ES
dc.language.isoen_USes_ES
dc.sourceClinical Microbiology Infection 2(1): 55-58es_ES
dc.subjectShigella sonnetes_ES
dc.subjectdiagnosises_ES
dc.subjectmonoclonal antibodyes_ES
dc.subjectimmunomagnetic separationes_ES
dc.subjectpolymerase chain reactiones_ES
dc.subjectSalud públicaes_ES
dc.titleRapid and sensitive detection of Shigella sonnei in feces by the use of an O-antigen-specific monoclonal antibody in a combined immunomagnetic separation-polymerase chain reaction assayes_ES
dc.typeinfo:eu-repo/semantics/reportes_ES
dc.typeReporte técnicoes_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones en Salud (INISA)es_ES


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