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dc.creatorRobertson, Nahid G.
dc.creatorUmang, Khetarpal
dc.creatorGutiérrez Espeleta, Gustavo A.
dc.creatorBieber, Frederick R.
dc.creatorMorton, Cynthia C.
dc.date.accessioned2015-08-03T17:47:02Z
dc.date.available2015-08-03T17:47:02Z
dc.date.issued1994
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0888754384714571#es_ES
dc.identifier.issn0888-7543
dc.identifier.issn1089-8646
dc.identifier.urihttp://hdl.handle.net/10669/15162
dc.descriptionArtículo científico -- Universidad de Costa Rica. Instituto de Investigaciones en Salud, 1994. Este documento es privado debido a las políticas de la revista en la que fue publicado.es_ES
dc.description.abstractWe used a combination of subtractive hybridization and differential screening strategies to identify genes that may function normally in hearing and, when mutated, result in deafness. A human fetal cochlear (membranous labyrinth) cDNA library was subtracted against total human fetal brain RNAs by an avidin-biotin-based procedure to enrich for cochlear transcripts. Subtracted cochlear clones were differentially screened with 32P-labeled total cochlear and total brain cDNA probes. Sequence analysis of clones that hybridized more intensely with cochlear than with brain cDNA probes revealed some previously characterized genes, including mitochondrial sequences, collagen type I alpha-2 (COL1A2), collagen type II alpha-1 (COL2A1), collagen type III alpha-1 (COL3A1), spermidine/spermine N1-acetyltransferase (SAT), osteonectin (SPARC), and peripheral myelin protein 22 (PMP22). Also identified were clones that are potential novel cochlear genes. Northern blots of cochlear and brain RNAs probed with COL1A2, COL2A1, COL3A1, SAT, SPARC, PMP22, and a novel sequence, designated Coch-5B2, confirm results of the subtractive procedure by showing preferential cochlear expression. A number of these genes serve structural or regulatory functions in extracellular matrix or neural conduction; defects in some of these genes are associated with disorders involving hearing loss. Partial sequence analysis of Coch-5B2 reveals a von Willebrand factor type A-like domain in this cDNA. To assess the cochlear specificity of Coch-5B2, a Northern blot panel of 14 human fetal tissue RNAs was probed with Coch-5B2, showing differential expression of this novel gene in the cochlea.es_ES
dc.description.sponsorshipUniversidad de Costa Rica, Instituto de Investigaciones en Saludes_ES
dc.language.isoen_USes_ES
dc.sourceGenomics 23 (1): 42-50es_ES
dc.subjectscreening strategieses_ES
dc.subjectfetales_ES
dc.subjectsubtractive hybridizationes_ES
dc.subjecthuman fetal cochleares_ES
dc.subjectSalud públicaes_ES
dc.subjectHuman geneticses_ES
dc.titleInsolation of Novel and Known Genes from a Human Fetal Cochlear cDNA Library Using Subtractive Hybridization and Differential Screeninges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doidoi:10.1006/geno.1994.1457
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones en Salud (INISA)es_ES


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