Replication of Influenza A and B Viruses in Human Diploid Cells
artículo original
Date
1983-09-20Author
Herrero Uribe, Libia
Mann, G.F.
Zuckerman, A.J.
Hockley, D.
Oxford, J.S.
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Show full item recordAbstract
Under optimal conditions, of high multiplicities of infection and with trypsin
included in the medium throughout the incubation period, high yields of infectious
influenza A and B viruses (106-5 p.f.u./ml) and of antigenically active haemagglutinin
(HA) (1 ug/HA/106 cells) were produced in human diploid MRC-5 cells. Budding virus
particles were seen as spherical or short rod-like protrusions on the surface of the
infected cells, and also on cell filopodia. Virus-induced cytoplasmic and nuclear
inclusions were present in infected cells. This virus—human cell system may be suitable
for studies of influenza virus persistence and for production of immunologically active
HA antigen.
Artículo científico -- Universidad de Costa Rica, Instituto de Investigaciones en Salud.1983
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